---
_id: '8607'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily
    conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein
    complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the
    recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails.
    However, in plants, very little is known on how PM proteins are sorted for CME
    and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid
    (BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin
    and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit,
    AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed
    tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in
    Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently,
    plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates
    that AP-2-dependent internalization of PM proteins via the recognition of functional
    tyrosine motifs also operates in plants.
article_processing_charge: No
article_type: original
author:
- first_name: D
  full_name: Liu, D
  last_name: Liu
- first_name: R
  full_name: Kumar, R
  last_name: Kumar
- first_name: Claus
  full_name: LAN, Claus
  last_name: LAN
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: W
  full_name: Siao, W
  last_name: Siao
- first_name: I
  full_name: Vanhoutte, I
  last_name: Vanhoutte
- first_name: P
  full_name: Wang, P
  last_name: Wang
- first_name: KW
  full_name: Bender, KW
  last_name: Bender
- first_name: K
  full_name: Yperman, K
  last_name: Yperman
- first_name: S
  full_name: Martins, S
  last_name: Martins
- first_name: X
  full_name: Zhao, X
  last_name: Zhao
- first_name: G
  full_name: Vert, G
  last_name: Vert
- first_name: D
  full_name: Van Damme, D
  last_name: Van Damme
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: E
  full_name: Russinova, E
  last_name: Russinova
citation:
  ama: Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is
    partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. 2020;32(11):3598-3612.
    doi:<a href="https://doi.org/10.1105/tpc.20.00384">10.1105/tpc.20.00384</a>
  apa: Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova,
    E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical
    tyrosine-based Motif. <i>Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.20.00384">https://doi.org/10.1105/tpc.20.00384</a>
  chicago: Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P
    Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by
    a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>. American Society of Plant
    Biologists, 2020. <a href="https://doi.org/10.1105/tpc.20.00384">https://doi.org/10.1105/tpc.20.00384</a>.
  ieee: D. Liu <i>et al.</i>, “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly
    driven by a canonical tyrosine-based Motif,” <i>Plant Cell</i>, vol. 32, no. 11.
    American Society of Plant Biologists, pp. 3598–3612, 2020.
  ista: Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K,
    Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020.
    Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based
    Motif. Plant Cell. 32(11), 3598–3612.
  mla: Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven
    by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>, vol. 32, no. 11, American
    Society of Plant Biologists, 2020, pp. 3598–612, doi:<a href="https://doi.org/10.1105/tpc.20.00384">10.1105/tpc.20.00384</a>.
  short: D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K.
    Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova,
    Plant Cell 32 (2020) 3598–3612.
date_created: 2020-10-05T12:45:16Z
date_published: 2020-11-01T00:00:00Z
date_updated: 2026-06-18T19:35:07Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.20.00384
ec_funded: 1
external_id:
  isi:
  - '000600226800021'
  pmid:
  - '32958564'
intvolume: '        32'
isi: 1
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://europepmc.org/article/MED/32958564
month: '11'
oa: 1
oa_version: Published Version
page: 3598-3612
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Cell
publication_identifier:
  eissn:
  - 1532-298x
  issn:
  - 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical
  tyrosine-based Motif
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2020'
...