article published yes Magdalena K. Sznurkowska author Edouard B Hannezo author 3A9DB764-F248-11E8-B48F-1D18A9856A870000-0001-6005-1561 Roberta Azzarelli author Lemonia Chatzeli author Tatsuro Ikeda author Shosei Yoshida author Anna Philpott author Benjamin D Simons author EdHa department Pancreatic islets play an essential role in regulating blood glucose level. Although the molecular pathways underlying islet cell differentiation are beginning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear. By combining unbiased and targeted lineage tracing, we address the events leading to islet formation in the mouse. From the statistical analysis of clones induced at multiple embryonic timepoints, here we show that, during the secondary transition, islet formation involves the aggregation of multiple equipotent endocrine progenitors that transition from a phase of stochastic amplification by cell division into a phase of sublineage restriction and limited islet fission. Together, these results explain quantitatively the heterogeneous size distribution and degree of polyclonality of maturing islets, as well as dispersion of progenitors within and between islets. Further, our results show that, during the secondary transition, α- and β-cells are generated in a contemporary manner. Together, these findings provide insight into the cellular basis of islet development. https://research-explorer.ista.ac.at/download/8669/8677/2020_NatureComm_Sznurkowska.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ Springer Nature2020 eng 2041-1723 33028844 00057724460000310.1038/s41467-020-18837-3 11 M. K. Sznurkowska <i>et al.</i>, “Tracing the cellular basis of islet specification in mouse pancreas,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020. Sznurkowska, Magdalena K., et al. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>, vol. 11, 5037, Springer Nature, 2020, doi:<a href="https://doi.org/10.1038/s41467-020-18837-3">10.1038/s41467-020-18837-3</a>. Sznurkowska MK, Hannezo EB, Azzarelli R, Chatzeli L, Ikeda T, Yoshida S, Philpott A, Simons BD. 2020. Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. 11, 5037. M.K. Sznurkowska, E.B. Hannezo, R. Azzarelli, L. Chatzeli, T. Ikeda, S. Yoshida, A. Philpott, B.D. Simons, Nature Communications 11 (2020). Sznurkowska, M. K., Hannezo, E. B., Azzarelli, R., Chatzeli, L., Ikeda, T., Yoshida, S., … Simons, B. D. (2020). Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-020-18837-3">https://doi.org/10.1038/s41467-020-18837-3</a> Sznurkowska, Magdalena K., Edouard B Hannezo, Roberta Azzarelli, Lemonia Chatzeli, Tatsuro Ikeda, Shosei Yoshida, Anna Philpott, and Benjamin D Simons. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>. Springer Nature, 2020. <a href="https://doi.org/10.1038/s41467-020-18837-3">https://doi.org/10.1038/s41467-020-18837-3</a>. Sznurkowska MK, Hannezo EB, Azzarelli R, et al. Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. 2020;11. doi:<a href="https://doi.org/10.1038/s41467-020-18837-3">10.1038/s41467-020-18837-3</a> 86692020-10-18T22:01:35Z2026-04-02T14:29:58Z