---
_id: '8813'
abstract:
- lang: eng
  text: 'In mammals, chromatin marks at imprinted genes are asymmetrically inherited
    to control parentally-biased gene expression. This control is thought predominantly
    to involve parent-specific differentially methylated regions (DMR) in genomic
    DNA. However, neither parent-of-origin-specific transcription nor DMRs have been
    comprehensively mapped. We here address this by integrating transcriptomic and
    epigenomic approaches in mouse preimplantation embryos (blastocysts). Transcriptome-analysis
    identified 71 genes expressed with previously unknown parent-of-origin-specific
    expression in blastocysts (nBiX: novel blastocyst-imprinted expression). Uniparental
    expression of nBiX genes disappeared soon after implantation. Micro-whole-genome
    bisulfite sequencing (μWGBS) of individual uniparental blastocysts detected 859
    DMRs. Only 18% of nBiXs were associated with a DMR, whereas 60% were associated
    with parentally-biased H3K27me3. This suggests a major role for Polycomb-mediated
    imprinting in blastocysts. Five nBiX-clusters contained at least one known imprinted
    gene, and five novel clusters contained exclusively nBiX-genes. These data suggest
    a complex program of stage-specific imprinting involving different tiers of regulation.'
article_processing_charge: No
author:
- first_name: Laura
  full_name: Santini, Laura
  last_name: Santini
- first_name: Florian
  full_name: Halbritter, Florian
  last_name: Halbritter
- first_name: Fabian
  full_name: Titz-Teixeira, Fabian
  last_name: Titz-Teixeira
- first_name: Toru
  full_name: Suzuki, Toru
  last_name: Suzuki
- first_name: Maki
  full_name: Asami, Maki
  last_name: Asami
- first_name: Julia
  full_name: Ramesmayer, Julia
  last_name: Ramesmayer
- first_name: Xiaoyan
  full_name: Ma, Xiaoyan
  last_name: Ma
- first_name: Andreas
  full_name: Lackner, Andreas
  last_name: Lackner
- first_name: Nick
  full_name: Warr, Nick
  last_name: Warr
- first_name: Florian
  full_name: Pauler, Florian
  id: 48EA0138-F248-11E8-B48F-1D18A9856A87
  last_name: Pauler
  orcid: 0000-0002-7462-0048
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Ernest
  full_name: Laue, Ernest
  last_name: Laue
- first_name: Matthias
  full_name: Farlik, Matthias
  last_name: Farlik
- first_name: Christoph
  full_name: Bock, Christoph
  last_name: Bock
- first_name: Andreas
  full_name: Beyer, Andreas
  last_name: Beyer
- first_name: Anthony C. F.
  full_name: Perry, Anthony C. F.
  last_name: Perry
- first_name: Martin
  full_name: Leeb, Martin
  last_name: Leeb
citation:
  ama: Santini L, Halbritter F, Titz-Teixeira F, et al. Novel imprints in mouse blastocysts
    are predominantly DNA methylation independent. <i>bioRxiv</i>. doi:<a href="https://doi.org/10.1101/2020.11.03.366948">10.1101/2020.11.03.366948</a>
  apa: Santini, L., Halbritter, F., Titz-Teixeira, F., Suzuki, T., Asami, M., Ramesmayer,
    J., … Leeb, M. (n.d.). Novel imprints in mouse blastocysts are predominantly DNA
    methylation independent. <i>bioRxiv</i>. <a href="https://doi.org/10.1101/2020.11.03.366948">https://doi.org/10.1101/2020.11.03.366948</a>
  chicago: Santini, Laura, Florian Halbritter, Fabian Titz-Teixeira, Toru Suzuki,
    Maki Asami, Julia Ramesmayer, Xiaoyan Ma, et al. “Novel Imprints in Mouse Blastocysts
    Are Predominantly DNA Methylation Independent.” <i>BioRxiv</i>, n.d. <a href="https://doi.org/10.1101/2020.11.03.366948">https://doi.org/10.1101/2020.11.03.366948</a>.
  ieee: L. Santini <i>et al.</i>, “Novel imprints in mouse blastocysts are predominantly
    DNA methylation independent,” <i>bioRxiv</i>. .
  ista: Santini L, Halbritter F, Titz-Teixeira F, Suzuki T, Asami M, Ramesmayer J,
    Ma X, Lackner A, Warr N, Pauler F, Hippenmeyer S, Laue E, Farlik M, Bock C, Beyer
    A, Perry ACF, Leeb M. Novel imprints in mouse blastocysts are predominantly DNA
    methylation independent. bioRxiv, <a href="https://doi.org/10.1101/2020.11.03.366948">10.1101/2020.11.03.366948</a>.
  mla: Santini, Laura, et al. “Novel Imprints in Mouse Blastocysts Are Predominantly
    DNA Methylation Independent.” <i>BioRxiv</i>, doi:<a href="https://doi.org/10.1101/2020.11.03.366948">10.1101/2020.11.03.366948</a>.
  short: L. Santini, F. Halbritter, F. Titz-Teixeira, T. Suzuki, M. Asami, J. Ramesmayer,
    X. Ma, A. Lackner, N. Warr, F. Pauler, S. Hippenmeyer, E. Laue, M. Farlik, C.
    Bock, A. Beyer, A.C.F. Perry, M. Leeb, BioRxiv (n.d.).
das_tickbox: '1'
date_created: 2020-11-26T07:17:19Z
date_published: 2020-11-05T00:00:00Z
date_updated: 2026-07-06T12:45:07Z
day: '05'
department:
- _id: SiHi
doi: 10.1101/2020.11.03.366948
external_id:
  pmid:
  - 'PPR234457 '
fulldoi: https://doi.org/10.1101/2020.11.03.366948
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2020.11.03.366948
month: '11'
oa: 1
oa_version: Preprint
pmid: 1
publication: bioRxiv
publication_status: submitted
status: public
title: Novel imprints in mouse blastocysts are predominantly DNA methylation independent
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...