---
res:
  bibo_abstract:
  - 'Maintaining fertility in a fluctuating environment is key to the reproductive
    success of flowering plants. Meiosis and pollen formation are particularly sensitive
    to changes in growing conditions, especially temperature. We have previously identified
    cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent
    meiosis and this may involve the regulation of alternative splicing (AS), including
    of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing
    two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain
    which may be involved in co-factor interaction. In leaves, both isoforms have
    distinct temperature-dependent functions on target mRNA processing, but their
    role in pollen development is unknown. In the present study, we characterize the
    role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the
    long (L) form is necessary and sufficient to rescue the fertility defects of the
    cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the
    other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of
    the ATP binding pocket of the kinase indicates that kinase activity is necessary
    for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize
    to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to
    rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore,
    we show that there is partial functional overlap between CDKG1 and its paralog
    CDKG2 that could in part be explained by overlapping gene expression.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Candida
      foaf_name: Nibau, Candida
      foaf_surname: Nibau
  - foaf_Person:
      foaf_givenName: Despoina
      foaf_name: Dadarou, Despoina
      foaf_surname: Dadarou
  - foaf_Person:
      foaf_givenName: Nestoras
      foaf_name: Kargios, Nestoras
      foaf_surname: Kargios
  - foaf_Person:
      foaf_givenName: Areti
      foaf_name: Mallioura, Areti
      foaf_surname: Mallioura
  - foaf_Person:
      foaf_givenName: Narcis
      foaf_name: Fernandez-Fuentes, Narcis
      foaf_surname: Fernandez-Fuentes
  - foaf_Person:
      foaf_givenName: Nicola
      foaf_name: Cavallari, Nicola
      foaf_surname: Cavallari
      foaf_workInfoHomepage: http://www.librecat.org/personId=457160E6-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: John H.
      foaf_name: Doonan, John H.
      foaf_surname: Doonan
  bibo_doi: 10.3389/fpls.2020.586870
  bibo_volume: 11
  dct_date: 2020^xs_gYear
  dct_identifier:
  - UT:000591637000001
  dct_isPartOf:
  - http://id.crossref.org/issn/1664-462X
  dct_language: eng
  dct_publisher: Frontiers@
  dct_title: A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1)
    to maintain fertility at high ambient temperature in Arabidopsis@
  fabio_hasPubmedId: '33240303'
...