---
_id: '8924'
abstract:
- lang: eng
text: 'Maintaining fertility in a fluctuating environment is key to the reproductive
success of flowering plants. Meiosis and pollen formation are particularly sensitive
to changes in growing conditions, especially temperature. We have previously identified
cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent
meiosis and this may involve the regulation of alternative splicing (AS), including
of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing
two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain
which may be involved in co-factor interaction. In leaves, both isoforms have
distinct temperature-dependent functions on target mRNA processing, but their
role in pollen development is unknown. In the present study, we characterize the
role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the
long (L) form is necessary and sufficient to rescue the fertility defects of the
cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the
other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of
the ATP binding pocket of the kinase indicates that kinase activity is necessary
for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize
to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to
rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore,
we show that there is partial functional overlap between CDKG1 and its paralog
CDKG2 that could in part be explained by overlapping gene expression.'
acknowledgement: CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1).
NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program
of the Austrian Federal Ministry of Science and Research and the City of Vienna.
article_number: '586870'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: Nestoras
full_name: Kargios, Nestoras
last_name: Kargios
- first_name: Areti
full_name: Mallioura, Areti
last_name: Mallioura
- first_name: Narcis
full_name: Fernandez-Fuentes, Narcis
last_name: Fernandez-Fuentes
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: John H.
full_name: Doonan, John H.
last_name: Doonan
citation:
ama: Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. 2020;11. doi:10.3389/fpls.2020.586870
apa: Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N.,
Cavallari, N., & Doonan, J. H. (2020). A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2020.586870
chicago: Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis
Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase
Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High
Ambient Temperature in Arabidopsis.” Frontiers in Plant Science. Frontiers,
2020. https://doi.org/10.3389/fpls.2020.586870.
ieee: C. Nibau et al., “A functional kinase is necessary for cyclin-dependent
kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,”
Frontiers in Plant Science, vol. 11. Frontiers, 2020.
ista: Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari
N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase
G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers
in Plant Science. 11, 586870.
mla: Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent
Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.”
Frontiers in Plant Science, vol. 11, 586870, Frontiers, 2020, doi:10.3389/fpls.2020.586870.
short: C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N.
Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).
date_created: 2020-12-06T23:01:14Z
date_published: 2020-11-10T00:00:00Z
date_updated: 2025-06-12T07:02:22Z
day: '10'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2020.586870
external_id:
isi:
- '000591637000001'
pmid:
- '33240303'
file:
- access_level: open_access
checksum: 1c0ee6ce9950aa665d6a5cc64aa6b752
content_type: application/pdf
creator: dernst
date_created: 2020-12-09T09:14:19Z
date_updated: 2020-12-09T09:14:19Z
file_id: '8929'
file_name: 2020_Frontiers_Nibau.pdf
file_size: 1833244
relation: main_file
success: 1
file_date_updated: 2020-12-09T09:14:19Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to
maintain fertility at high ambient temperature in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2020'
...