article published Elena Alkalaeva author Boris Eliseev author Alexandre Ambrogelly author Peter Vlasov author Fyodor Kondrashov author 44FDEF62-F248-11E8-B48F-1D18A9856A870000-0001-8243-4694 Sarath Gundllapalli author Ludmila Frolova author Dieter Söll author Lev Kisselev author Although some data link archaeal and eukaryotic translation, the overall mechanism of protein synthesis in archaea remains largely obscure. Both archaeal (aRF1) and eukaryotic (eRF1) single release factors recognize all three stop codons. The archaeal genus Methanosarcinaceae contains two aRF1 homologs, and also uses the UAG stop to encode the 22nd amino acid, pyrrolysine. Here we provide an analysis of the last stage of archaeal translation in pyrrolysine-utilizing species. We demonstrated that only one of two Methanosarcina barkeri aRF1 homologs possesses activity and recognizes all three stop codons. The second aRF1 homolog may have another unknown function. The mechanism of pyrrolysine incorporation in the Methanosarcinaceae is discussed. Elsevier2009 10.1016/j.febslet.2009.09.044 583213455 - 3460 yes E. Alkalaeva, B. Eliseev, A. Ambrogelly, P. Vlasov, F. Kondrashov, S. Gundllapalli, L. Frolova, D. Söll, L. Kisselev, FEBS Letters 583 (2009) 3455–3460. Alkalaeva, E., Eliseev, B., Ambrogelly, A., Vlasov, P., Kondrashov, F., Gundllapalli, S., … Kisselev, L. (2009). Translation termination in pyrrolysine-utilizing archaea. <i>FEBS Letters</i>. Elsevier. <a href="https://doi.org/10.1016/j.febslet.2009.09.044">https://doi.org/10.1016/j.febslet.2009.09.044</a> Alkalaeva E, Eliseev B, Ambrogelly A, et al. Translation termination in pyrrolysine-utilizing archaea. <i>FEBS Letters</i>. 2009;583(21):3455-3460. doi:<a href="https://doi.org/10.1016/j.febslet.2009.09.044">10.1016/j.febslet.2009.09.044</a> Alkalaeva, Elena, et al. “Translation Termination in Pyrrolysine-Utilizing Archaea.” <i>FEBS Letters</i>, vol. 583, no. 21, Elsevier, 2009, pp. 3455–60, doi:<a href="https://doi.org/10.1016/j.febslet.2009.09.044">10.1016/j.febslet.2009.09.044</a>. Alkalaeva E, Eliseev B, Ambrogelly A, Vlasov P, Kondrashov F, Gundllapalli S, Frolova L, Söll D, Kisselev L. 2009. Translation termination in pyrrolysine-utilizing archaea. FEBS Letters. 583(21), 3455–3460. Alkalaeva, Elena, Boris Eliseev, Alexandre Ambrogelly, Peter Vlasov, Fyodor Kondrashov, Sarath Gundllapalli, Ludmila Frolova, Dieter Söll, and Lev Kisselev. “Translation Termination in Pyrrolysine-Utilizing Archaea.” <i>FEBS Letters</i>. Elsevier, 2009. <a href="https://doi.org/10.1016/j.febslet.2009.09.044">https://doi.org/10.1016/j.febslet.2009.09.044</a>. E. Alkalaeva <i>et al.</i>, “Translation termination in pyrrolysine-utilizing archaea,” <i>FEBS Letters</i>, vol. 583, no. 21. Elsevier, pp. 3455–3460, 2009. 9082018-12-11T11:49:08Z2021-01-12T08:21:49Z