---
_id: '9094'
abstract:
- lang: eng
  text: Dendritic cells (DCs) are crucial for the priming of naive T cells and the
    initiation of adaptive immunity. Priming is initiated at a heterologous cell–cell
    contact, the immunological synapse (IS). While it is established that F-actin
    dynamics regulates signaling at the T cell side of the contact, little is known
    about the cytoskeletal contribution on the DC side. Here, we show that the DC
    actin cytoskeleton is decisive for the formation of a multifocal synaptic structure,
    which correlates with T cell priming efficiency. DC actin at the IS appears in
    transient foci that are dynamized by the WAVE regulatory complex (WRC). The absence
    of the WRC in DCs leads to stabilized contacts with T cells, caused by an increase
    in ICAM1-integrin–mediated cell–cell adhesion. This results in lower numbers of
    activated and proliferating T cells, demonstrating an important role for DC actin
    in the regulation of immune synapse functionality.
article_number: e202006081
article_processing_charge: No
article_type: original
author:
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: LM
  full_name: Altenburger, LM
  last_name: Altenburger
- first_name: R
  full_name: Hauschild, R
  last_name: Hauschild
- first_name: Frank P
  full_name: Assen, Frank P
  id: 3A8E7F24-F248-11E8-B48F-1D18A9856A87
  last_name: Assen
  orcid: 0000-0003-3470-6119
- first_name: K
  full_name: Rottner, K
  last_name: Rottner
- first_name: Stradal
  full_name: TEB, Stradal
  last_name: TEB
- first_name: A
  full_name: Diz-Muñoz, A
  last_name: Diz-Muñoz
- first_name: JV
  full_name: Stein, JV
  last_name: Stein
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Leithner AF, Altenburger L, Hauschild R, et al. Dendritic cell actin dynamics
    control contact duration and priming efficiency at the immunological synapse.
    <i>Journal of Cell Biology</i>. 2021;220(4). doi:<a href="https://doi.org/10.1083/jcb.202006081">10.1083/jcb.202006081</a>
  apa: Leithner, A. F., Altenburger, L., Hauschild, R., Assen, F. P., Rottner, K.,
    TEB, S., … Sixt, M. K. (2021). Dendritic cell actin dynamics control contact duration
    and priming efficiency at the immunological synapse. <i>Journal of Cell Biology</i>.
    Rockefeller University Press. <a href="https://doi.org/10.1083/jcb.202006081">https://doi.org/10.1083/jcb.202006081</a>
  chicago: Leithner, Alexander F, LM Altenburger, R Hauschild, Frank P Assen, K Rottner,
    Stradal TEB, A Diz-Muñoz, JV Stein, and Michael K Sixt. “Dendritic Cell Actin
    Dynamics Control Contact Duration and Priming Efficiency at the Immunological
    Synapse.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2021.
    <a href="https://doi.org/10.1083/jcb.202006081">https://doi.org/10.1083/jcb.202006081</a>.
  ieee: A. F. Leithner <i>et al.</i>, “Dendritic cell actin dynamics control contact
    duration and priming efficiency at the immunological synapse,” <i>Journal of Cell
    Biology</i>, vol. 220, no. 4. Rockefeller University Press, 2021.
  ista: Leithner AF, Altenburger L, Hauschild R, Assen FP, Rottner K, TEB S, Diz-Muñoz
    A, Stein J, Sixt MK. 2021. Dendritic cell actin dynamics control contact duration
    and priming efficiency at the immunological synapse. Journal of Cell Biology.
    220(4), e202006081.
  mla: Leithner, Alexander F., et al. “Dendritic Cell Actin Dynamics Control Contact
    Duration and Priming Efficiency at the Immunological Synapse.” <i>Journal of Cell
    Biology</i>, vol. 220, no. 4, e202006081, Rockefeller University Press, 2021,
    doi:<a href="https://doi.org/10.1083/jcb.202006081">10.1083/jcb.202006081</a>.
  short: A.F. Leithner, L. Altenburger, R. Hauschild, F.P. Assen, K. Rottner, S. TEB,
    A. Diz-Muñoz, J. Stein, M.K. Sixt, Journal of Cell Biology 220 (2021).
corr_author: '1'
date_created: 2021-02-05T10:08:04Z
date_published: 2021-04-05T00:00:00Z
date_updated: 2024-10-09T21:00:23Z
day: '05'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1083/jcb.202006081
external_id:
  isi:
  - '000626365700001'
  pmid:
  - '33533935'
file:
- access_level: open_access
  checksum: 843ebc153847c8626e13c9c5ce71d533
  content_type: application/pdf
  creator: dernst
  date_created: 2022-05-12T14:16:21Z
  date_updated: 2022-05-12T14:16:21Z
  file_id: '11367'
  file_name: 2021_JournCellBiology_Leithner.pdf
  file_size: 5102328
  relation: main_file
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file_date_updated: 2022-05-12T14:16:21Z
has_accepted_license: '1'
intvolume: '       220'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
  eissn:
  - 1540-8140
  issn:
  - 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dendritic cell actin dynamics control contact duration and priming efficiency
  at the immunological synapse
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
    BY-NC-SA 4.0)
  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 220
year: '2021'
...