---
res:
  bibo_abstract:
  - Cryo-EM grid preparation is an important bottleneck in protein structure determination,
    especially for membrane proteins, typically requiring screening of a large number
    of conditions. We systematically investigated the effects of buffer components,
    blotting conditions and grid types on the outcome of grid preparation of five
    different membrane protein samples. Aggregation was the most common type of problem
    which was addressed by changing detergents, salt concentration or reconstitution
    of proteins into nanodiscs or amphipols. We show that the optimal concentration
    of detergent is between 0.05 and 0.4% and that the presence of a low concentration
    of detergent with a high critical micellar concentration protects the proteins
    from denaturation at the air-water interface. Furthermore, we discuss the strategies
    for achieving an adequate ice thickness, particle coverage and orientation distribution
    on free ice and on support films. Our findings provide a clear roadmap for comprehensive
    screening of conditions for cryo-EM grid preparation of membrane proteins.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Domen
      foaf_name: Kampjut, Domen
      foaf_surname: Kampjut
      foaf_workInfoHomepage: http://www.librecat.org/personId=37233050-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-6018-3422
  - foaf_Person:
      foaf_givenName: Julia
      foaf_name: Steiner, Julia
      foaf_surname: Steiner
      foaf_workInfoHomepage: http://www.librecat.org/personId=3BB67EB0-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-0493-3775
  - foaf_Person:
      foaf_givenName: Leonid A
      foaf_name: Sazanov, Leonid A
      foaf_surname: Sazanov
      foaf_workInfoHomepage: http://www.librecat.org/personId=338D39FE-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-0977-7989
  bibo_doi: 10.1016/j.isci.2021.102139
  bibo_issue: '3'
  bibo_volume: 24
  dct_date: 2021^xs_gYear
  dct_identifier:
  - UT:000631646000012
  dct_isPartOf:
  - http://id.crossref.org/issn/2589-0042
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Cryo-EM grid optimization for membrane proteins@
  fabio_hasPubmedId: '33665558'
...