@article{923, abstract = {The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP SAT, demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues.}, author = {Fré, Silvia and Hannezo, Edouard B and Šale, Sanja and Huyghe, Mathilde and Lafkas, Daniel and Kissel, Holger and Louvi, Angeliki and Greve, Jeffrey and Louvard, Daniel and Artavanis Tsakonas, Spyros}, journal = {PLoS One}, number = {10}, publisher = {Public Library of Science}, title = {{Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice}}, doi = {10.1371/journal.pone.0025785}, volume = {6}, year = {2011}, }