{"_id":"923","extern":"1","file":[{"date_created":"2019-05-10T11:20:26Z","date_updated":"2020-07-14T12:48:15Z","creator":"dernst","access_level":"open_access","file_size":2860615,"content_type":"application/pdf","file_id":"6401","relation":"main_file","file_name":"2011_PLOS1_Fre.PDF","checksum":"b4e864125dfcb9fa57a9e01688838081"}],"status":"public","doi":"10.1371/journal.pone.0025785","oa_version":"Published Version","publist_id":"6520","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:21:56Z","type":"journal_article","file_date_updated":"2020-07-14T12:48:15Z","issue":"10","publication":"PLoS One","publisher":"Public Library of Science","date_created":"2018-12-11T11:49:13Z","month":"10","publication_status":"published","quality_controlled":"1","volume":6,"date_published":"2011-10-03T00:00:00Z","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"article_number":"e25785","title":"Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice","citation":{"ieee":"S. Fré <i>et al.</i>, “Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice,” <i>PLoS One</i>, vol. 6, no. 10. Public Library of Science, 2011.","ama":"Fré S, Hannezo EB, Šale S, et al. Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. <i>PLoS One</i>. 2011;6(10). doi:<a href=\"https://doi.org/10.1371/journal.pone.0025785\">10.1371/journal.pone.0025785</a>","apa":"Fré, S., Hannezo, E. B., Šale, S., Huyghe, M., Lafkas, D., Kissel, H., … Artavanis Tsakonas, S. (2011). Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0025785\">https://doi.org/10.1371/journal.pone.0025785</a>","chicago":"Fré, Silvia, Edouard B Hannezo, Sanja Šale, Mathilde Huyghe, Daniel Lafkas, Holger Kissel, Angeliki Louvi, Jeffrey Greve, Daniel Louvard, and Spyros Artavanis Tsakonas. “Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock in Mice.” <i>PLoS One</i>. Public Library of Science, 2011. <a href=\"https://doi.org/10.1371/journal.pone.0025785\">https://doi.org/10.1371/journal.pone.0025785</a>.","short":"S. Fré, E.B. Hannezo, S. Šale, M. Huyghe, D. Lafkas, H. Kissel, A. Louvi, J. Greve, D. Louvard, S. Artavanis Tsakonas, PLoS One 6 (2011).","mla":"Fré, Silvia, et al. “Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock in Mice.” <i>PLoS One</i>, vol. 6, no. 10, e25785, Public Library of Science, 2011, doi:<a href=\"https://doi.org/10.1371/journal.pone.0025785\">10.1371/journal.pone.0025785</a>.","ista":"Fré S, Hannezo EB, Šale S, Huyghe M, Lafkas D, Kissel H, Louvi A, Greve J, Louvard D, Artavanis Tsakonas S. 2011. Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. PLoS One. 6(10), e25785."},"intvolume":"         6","ddc":["570"],"language":[{"iso":"eng"}],"day":"03","author":[{"full_name":"Fré, Silvia","first_name":"Silvia","last_name":"Fré"},{"first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Šale, Sanja","first_name":"Sanja","last_name":"Šale"},{"first_name":"Mathilde","last_name":"Huyghe","full_name":"Huyghe, Mathilde"},{"full_name":"Lafkas, Daniel","first_name":"Daniel","last_name":"Lafkas"},{"full_name":"Kissel, Holger","last_name":"Kissel","first_name":"Holger"},{"last_name":"Louvi","first_name":"Angeliki","full_name":"Louvi, Angeliki"},{"last_name":"Greve","first_name":"Jeffrey","full_name":"Greve, Jeffrey"},{"full_name":"Louvard, Daniel","last_name":"Louvard","first_name":"Daniel"},{"last_name":"Artavanis Tsakonas","first_name":"Spyros","full_name":"Artavanis Tsakonas, Spyros"}],"abstract":[{"lang":"eng","text":"The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP SAT, demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues."}],"oa":1,"year":"2011"}