---
_id: '9244'
abstract:
- lang: eng
text: 'Organ function depends on tissues adopting the correct architecture. However,
insights into organ architecture are currently hampered by an absence of standardized
quantitative 3D analysis. We aimed to develop a robust technology to visualize,
digitalize, and segment the architecture of two tubular systems in 3D: double
resin casting micro computed tomography (DUCT). As proof of principle, we applied
DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized
by intrahepatic bile duct paucity, that can spontaneously generate a biliary system
in adulthood. DUCT identified increased central biliary branching and peripheral
bile duct tortuosity as two compensatory processes occurring in distinct regions
of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume
and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis,
which can reveal novel phenotypes and provide a standardized method of defining
liver architecture in mouse models.'
acknowledgement: "Work in ERA lab is supported by the Swedish Research Council, the
Center of Innovative Medicine (CIMED) Grant, Karolinska Institutet, and the Heart
and Lung Foundation, and\r\nthe Daniel Alagille Award from the European Association
for the Study of the Liver. One project in ERA lab is funded by ModeRNA, unrelated
to this project. The funders have no role in the design or interpretation of the
work. SH has been supported by a KI-MU PhD student program, and by a Wera Ekstro¨m
Foundation Scholarship. We are grateful for support from Tornspiran foundation to
NVH. JK: This research was carried out under the project CEITEC 2020 (LQ1601) with
financial support from the Ministry of Education, Youth and Sports of the Czech
Republic under the National Sustainability Programme II and CzechNanoLab Research
Infrastructure supported by MEYS CR (LM2018110) . UL: The financial support from
the Swedish Research Council and ICMC (Integrated CardioMetabolic Center) is acknowledged.
JJ: The work was supported by the Grant Agency of Masaryk University (project no.
MUNI/A/1565/2018). We thank Kari Huppert and Stacey Huppert for their expertise
and help regarding bile duct cannulation and their laboratory hospitality. We also
thank Nadja Schultz and Charlotte L Mattsson for their help with common bile duct
cannulation. We thank Daniel Holl for his help with trachea cannulation. We thank
Nikos Papadogiannakis for his assistance with mild Alagille biopsy samples and discussion.
We thank Karolinska Biomedicum Imaging Core, especially Shigeaki Kanatani for his
help with image analysis. We thank Jan Masek and Carolina Gutierrez for their scientific
input in manuscript writing. We thank Peter Ranefall and the BioImage Informatics
(SciLife national facility) for their help writing parts of the MATLAB pipeline.\r\nThe
TROMA-III antibody developed by Rolf Kemler was obtained from the Developmental
Studies Hybridoma (DSHB) Bank developed under the auspices of NICHD and maintained
by The University of Iowa, Department of Biological Sciences, Iowa City, IA52242.
We thank Goncalo M Brito for all illustrations. This work was supported by the European
Union (European Research Council Starting grant 851288 to E.H.)."
article_number: e60916
article_processing_charge: No
article_type: original
author:
- first_name: Simona
full_name: Hankeova, Simona
last_name: Hankeova
- first_name: Jakub
full_name: Salplachta, Jakub
last_name: Salplachta
- first_name: Tomas
full_name: Zikmund, Tomas
last_name: Zikmund
- first_name: Michaela
full_name: Kavkova, Michaela
last_name: Kavkova
- first_name: Noémi
full_name: Van Hul, Noémi
last_name: Van Hul
- first_name: Adam
full_name: Brinek, Adam
last_name: Brinek
- first_name: Veronika
full_name: Smekalova, Veronika
last_name: Smekalova
- first_name: Jakub
full_name: Laznovsky, Jakub
last_name: Laznovsky
- first_name: Feven
full_name: Dawit, Feven
last_name: Dawit
- first_name: Josef
full_name: Jaros, Josef
last_name: Jaros
- first_name: Vítězslav
full_name: Bryja, Vítězslav
last_name: Bryja
- first_name: Urban
full_name: Lendahl, Urban
last_name: Lendahl
- first_name: Ewa
full_name: Ellis, Ewa
last_name: Ellis
- first_name: Antal
full_name: Nemeth, Antal
last_name: Nemeth
- first_name: Björn
full_name: Fischler, Björn
last_name: Fischler
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jozef
full_name: Kaiser, Jozef
last_name: Kaiser
- first_name: Emma Rachel
full_name: Andersson, Emma Rachel
last_name: Andersson
citation:
ama: Hankeova S, Salplachta J, Zikmund T, et al. DUCT reveals architectural mechanisms
contributing to bile duct recovery in a mouse model for alagille syndrome. eLife.
2021;10. doi:10.7554/eLife.60916
apa: Hankeova, S., Salplachta, J., Zikmund, T., Kavkova, M., Van Hul, N., Brinek,
A., … Andersson, E. R. (2021). DUCT reveals architectural mechanisms contributing
to bile duct recovery in a mouse model for alagille syndrome. ELife. eLife
Sciences Publications. https://doi.org/10.7554/eLife.60916
chicago: Hankeova, Simona, Jakub Salplachta, Tomas Zikmund, Michaela Kavkova, Noémi
Van Hul, Adam Brinek, Veronika Smekalova, et al. “DUCT Reveals Architectural Mechanisms
Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife.
eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.60916.
ieee: S. Hankeova et al., “DUCT reveals architectural mechanisms contributing
to bile duct recovery in a mouse model for alagille syndrome,” eLife, vol.
10. eLife Sciences Publications, 2021.
ista: Hankeova S, Salplachta J, Zikmund T, Kavkova M, Van Hul N, Brinek A, Smekalova
V, Laznovsky J, Dawit F, Jaros J, Bryja V, Lendahl U, Ellis E, Nemeth A, Fischler
B, Hannezo EB, Kaiser J, Andersson ER. 2021. DUCT reveals architectural mechanisms
contributing to bile duct recovery in a mouse model for alagille syndrome. eLife.
10, e60916.
mla: Hankeova, Simona, et al. “DUCT Reveals Architectural Mechanisms Contributing
to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife, vol.
10, e60916, eLife Sciences Publications, 2021, doi:10.7554/eLife.60916.
short: S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek,
V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis,
A. Nemeth, B. Fischler, E.B. Hannezo, J. Kaiser, E.R. Andersson, ELife 10 (2021).
date_created: 2021-03-14T23:01:34Z
date_published: 2021-02-26T00:00:00Z
date_updated: 2023-08-07T14:12:54Z
day: '26'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.7554/eLife.60916
ec_funded: 1
external_id:
isi:
- '000625357100001'
pmid:
- '33635272'
file:
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creator: dernst
date_created: 2021-03-22T08:50:33Z
date_updated: 2021-03-22T08:50:33Z
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file_name: 2021_eLife_Hankeova.pdf
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has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
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license: https://creativecommons.org/licenses/by/4.0/
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: eLife
publication_identifier:
eissn:
- 2050084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: DUCT reveals architectural mechanisms contributing to bile duct recovery in
a mouse model for alagille syndrome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...