--- res: bibo_abstract: - 'Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the root and refine the contributions of key players in this process. In addition, we determine the quantitative kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic insights into root development, the tools developed here will enable biologists to study kinetics and morphology of various critical processes at the single cell-level in whole organisms.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Yangjie foaf_name: Hu, Yangjie foaf_surname: Hu - foaf_Person: foaf_givenName: Moutasem foaf_name: Omary, Moutasem foaf_surname: Omary - foaf_Person: foaf_givenName: Yun foaf_name: Hu, Yun foaf_surname: Hu - foaf_Person: foaf_givenName: Ohad foaf_name: Doron, Ohad foaf_surname: Doron - foaf_Person: foaf_givenName: Lukas foaf_name: Hörmayer, Lukas foaf_surname: Hörmayer foaf_workInfoHomepage: http://www.librecat.org/personId=2EEE7A2A-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Qingguo foaf_name: Chen, Qingguo foaf_surname: Chen - foaf_Person: foaf_givenName: Or foaf_name: Megides, Or foaf_surname: Megides - foaf_Person: foaf_givenName: Ori foaf_name: Chekli, Ori foaf_surname: Chekli - foaf_Person: foaf_givenName: Zhaojun foaf_name: Ding, Zhaojun foaf_surname: Ding - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Yunde foaf_name: Zhao, Yunde foaf_surname: Zhao - foaf_Person: foaf_givenName: Ilan foaf_name: Tsarfaty, Ilan foaf_surname: Tsarfaty - foaf_Person: foaf_givenName: Eilon foaf_name: Shani, Eilon foaf_surname: Shani bibo_doi: 10.1038/s41467-021-21802-3 bibo_volume: 12 dct_date: 2021^xs_gYear dct_identifier: - UT:000630419400048 dct_isPartOf: - http://id.crossref.org/issn/20411723 dct_language: eng dct_publisher: Springer Nature@ dct_title: Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing@ fabio_hasPubmedId: '33712581' ...