--- res: bibo_abstract: - Gene expression levels are influenced by multiple coexisting molecular mechanisms. Some of these interactions such as those of transcription factors and promoters have been studied extensively. However, predicting phenotypes of gene regulatory networks (GRNs) remains a major challenge. Here, we use a well-defined synthetic GRN to study in Escherichia coli how network phenotypes depend on local genetic context, i.e. the genetic neighborhood of a transcription factor and its relative position. We show that one GRN with fixed topology can display not only quantitatively but also qualitatively different phenotypes, depending solely on the local genetic context of its components. Transcriptional read-through is the main molecular mechanism that places one transcriptional unit (TU) within two separate regulons without the need for complex regulatory sequences. We propose that relative order of individual TUs, with its potential for combinatorial complexity, plays an important role in shaping phenotypes of GRNs.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Anna A foaf_name: Nagy-Staron, Anna A foaf_surname: Nagy-Staron foaf_workInfoHomepage: http://www.librecat.org/personId=3ABC5BA6-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-1391-8377 - foaf_Person: foaf_givenName: Kathrin foaf_name: Tomasek, Kathrin foaf_surname: Tomasek foaf_workInfoHomepage: http://www.librecat.org/personId=3AEC8556-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-3768-877X - foaf_Person: foaf_givenName: Caroline foaf_name: Caruso Carter, Caroline foaf_surname: Caruso Carter - foaf_Person: foaf_givenName: Elisabeth foaf_name: Sonnleitner, Elisabeth foaf_surname: Sonnleitner - foaf_Person: foaf_givenName: Bor foaf_name: Kavcic, Bor foaf_surname: Kavcic foaf_workInfoHomepage: http://www.librecat.org/personId=350F91D2-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6041-254X - foaf_Person: foaf_givenName: Tiago foaf_name: Paixão, Tiago foaf_surname: Paixão - foaf_Person: foaf_givenName: Calin C foaf_name: Guet, Calin C foaf_surname: Guet foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6220-2052 bibo_doi: 10.7554/elife.65993 bibo_volume: 10 dct_date: 2021^xs_gYear dct_identifier: - UT:000631050900001 dct_isPartOf: - http://id.crossref.org/issn/2050-084X dct_language: eng dct_publisher: eLife Sciences Publications@ dct_subject: - Genetics and Molecular Biology dct_title: Local genetic context shapes the function of a gene regulatory network@ ...