AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells

article AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells published yes Matous Glanc author 1AE1EA24-02D0-11E9-9BAA-DAF4881429F20000-0003-0619-7783 K Van Gelderen author Lukas Hörmayer author 2EEE7A2A-F248-11E8-B48F-1D18A9856A870000-0001-8295-2926 Shutang Tan author 2DE75584-F248-11E8-B48F-1D18A9856A870000-0002-0471-8285 S Naramoto author Xixi Zhang author 61A66458-47E9-11EA-85BA-8AEAAF14E49A0000-0001-7048-4627 David Domjan author C684CD7A-257E-11EA-9B6F-D8588B4F947F0000-0003-2267-106X L Vcelarova author Robert Hauschild author 4E01D6B4-F248-11E8-B48F-1D18A9856A870000-0001-9843-3522 Alexander J Johnson author 46A62C3A-F248-11E8-B48F-1D18A9856A870000-0002-2739-8843 E de Koning author M van Dop author E Rademacher author S Janson author X Wei author Gergely Molnar author 34F1AF46-F248-11E8-B48F-1D18A9856A87 Matyas Fendrych author 43905548-F248-11E8-B48F-1D18A9856A870000-0002-9767-8699 B De Rybel author R Offringa author Jiří Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 JiFr department Tracing Evolution of Auxin Transport and Polarity in Plants project Molecular mechanisms of endocytic cargo recognition in plants project Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development. https://research-explorer.ista.ac.at/download/9290/9303/2021_CurrentBiology_Glanc.pdf application/pdfno Elsevier2021 eng Current Biology 0960-9822 1879-0445 33705718 00065307780000410.1016/j.cub.2021.02.028 3191918-1930 Glanc M, Van Gelderen K, Hörmayer L, Tan S, Naramoto S, Zhang X, Domjan D, Vcelarova L, Hauschild R, Johnson AJ, de Koning E, van Dop M, Rademacher E, Janson S, Wei X, Molnar G, Fendrych M, De Rybel B, Offringa R, Friml J. 2021. AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells. Current Biology. 31(9), 1918–1930. Glanc M, Van Gelderen K, Hörmayer L, et al. AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells. Current Biology. 2021;31(9):1918-1930. doi:<a href="https://doi.org/10.1016/j.cub.2021.02.028">10.1016/j.cub.2021.02.028</a> M. Glanc et al., “AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells,” Current Biology, vol. 31, no. 9. Elsevier, pp. 1918–1930, 2021. Glanc, Matous, et al. “AGC Kinases and MAB4/MEL Proteins Maintain PIN Polarity by Limiting Lateral Diffusion in Plant Cells.” Current Biology, vol. 31, no. 9, Elsevier, 2021, pp. 1918–30, doi:<a href="https://doi.org/10.1016/j.cub.2021.02.028">10.1016/j.cub.2021.02.028</a>. M. Glanc, K. Van Gelderen, L. Hörmayer, S. Tan, S. Naramoto, X. Zhang, D. Domjan, L. Vcelarova, R. Hauschild, A.J. Johnson, E. de Koning, M. van Dop, E. Rademacher, S. Janson, X. Wei, G. Molnar, M. Fendrych, B. De Rybel, R. Offringa, J. Friml, Current Biology 31 (2021) 1918–1930. Glanc, Matous, K Van Gelderen, Lukas Hörmayer, Shutang Tan, S Naramoto, Xixi Zhang, David Domjan, et al. “AGC Kinases and MAB4/MEL Proteins Maintain PIN Polarity by Limiting Lateral Diffusion in Plant Cells.” Current Biology. Elsevier, 2021. <a href="https://doi.org/10.1016/j.cub.2021.02.028">https://doi.org/10.1016/j.cub.2021.02.028</a>. Glanc, M., Van Gelderen, K., Hörmayer, L., Tan, S., Naramoto, S., Zhang, X., … Friml, J. (2021). AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells. Current Biology. Elsevier. <a href="https://doi.org/10.1016/j.cub.2021.02.028">https://doi.org/10.1016/j.cub.2021.02.028</a> 92902021-03-26T12:09:33Z2025-04-14T07:45:00Z