--- res: bibo_abstract: - Homeostatic replacement of epithelial cells from basal precursors is a multistep process involving progenitor cell specification, radial intercalation and, finally, apical surface emergence. Recent data demonstrate that actin-based pushing under the control of the formin protein Fmn1 drives apical emergence in nascent multiciliated epithelial cells (MCCs), but little else is known about this actin network or the control of Fmn1. Here, we explore the role of the small GTPase RhoA in MCC apical emergence. Disruption of RhoA function reduced the rate of apical surface expansion and decreased the final size of the apical domain. Analysis of cell shapes suggests that RhoA alters the balance of forces exerted on the MCC apical surface. Finally, quantitative time-lapse imaging and fluorescence recovery after photobleaching studies argue that RhoA works in concert with Fmn1 to control assembly of the specialized apical actin network in MCCs. These data provide new molecular insights into epithelial apical surface assembly and could also shed light on mechanisms of apical lumen formation@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jakub foaf_name: Sedzinski, Jakub foaf_surname: Sedzinski - foaf_Person: foaf_givenName: Edouard B foaf_name: Hannezo, Edouard B foaf_surname: Hannezo foaf_workInfoHomepage: http://www.librecat.org/personId=3A9DB764-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6005-1561 - foaf_Person: foaf_givenName: Fan foaf_name: Tu, Fan foaf_surname: Tu - foaf_Person: foaf_givenName: Maté foaf_name: Biro, Maté foaf_surname: Biro - foaf_Person: foaf_givenName: John foaf_name: Wallingford, John foaf_surname: Wallingford bibo_doi: 10.1242/jcs.202234 bibo_issue: '5' bibo_volume: 130 dct_date: 2017^xs_gYear dct_language: eng dct_publisher: Company of Biologists@ dct_title: RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells @ ...