---
OA_place: publisher
_id: '9397'
abstract:
- lang: eng
  text: Accumulation of interstitial fluid (IF) between embryonic cells is a common
    phenomenon in vertebrate embryogenesis. Unlike other model systems, where these
    accumulations coalesce into a large central cavity – the blastocoel, in zebrafish,
    IF is more uniformly distributed between the deep cells (DC) before the onset
    of gastrulation. This is likely due to the presence of a large extraembryonic
    structure – the yolk cell (YC) at the position where the blastocoel typically
    forms in other model organisms. IF has long been speculated to play a role in
    tissue morphogenesis during embryogenesis, but direct evidence supporting such
    function is still sparse. Here we show that the relocalization of IF to the interface
    between the YC and DC/epiblast is critical for axial mesendoderm (ME) cell protrusion
    formation and migration along this interface, a key process in embryonic axis
    formation. We further demonstrate that axial ME cell migration and IF relocalization
    engage in a positive feedback loop, where axial ME migration triggers IF accumulation
    ahead of the advancing axial ME tissue by mechanically compressing the overlying
    epiblast cell layer. Upon compression, locally induced flow relocalizes the IF
    through the porous epiblast tissue resulting in an IF accumulation ahead of the
    leading axial ME. This IF accumulation, in turn, promotes cell protrusion formation
    and migration of the leading axial ME cells, thereby facilitating axial ME extension.
    Our findings reveal a central role of dynamic IF relocalization in orchestrating
    germ layer morphogenesis during gastrulation.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
citation:
  ama: Huljev K. Coordinated spatiotemporal reorganization of interstitial fluid is
    required for axial mesendoderm migration in zebrafish gastrulation. 2021. doi:<a
    href="https://doi.org/10.15479/at:ista:9397">10.15479/at:ista:9397</a>
  apa: Huljev, K. (2021). <i>Coordinated spatiotemporal reorganization of interstitial
    fluid is required for axial mesendoderm migration in zebrafish gastrulation</i>.
    Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/at:ista:9397">https://doi.org/10.15479/at:ista:9397</a>
  chicago: Huljev, Karla. “Coordinated Spatiotemporal Reorganization of Interstitial
    Fluid Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation.”
    Institute of Science and Technology Austria, 2021. <a href="https://doi.org/10.15479/at:ista:9397">https://doi.org/10.15479/at:ista:9397</a>.
  ieee: K. Huljev, “Coordinated spatiotemporal reorganization of interstitial fluid
    is required for axial mesendoderm migration in zebrafish gastrulation,” Institute
    of Science and Technology Austria, 2021.
  ista: Huljev K. 2021. Coordinated spatiotemporal reorganization of interstitial
    fluid is required for axial mesendoderm migration in zebrafish gastrulation. Institute
    of Science and Technology Austria.
  mla: Huljev, Karla. <i>Coordinated Spatiotemporal Reorganization of Interstitial
    Fluid Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation</i>.
    Institute of Science and Technology Austria, 2021, doi:<a href="https://doi.org/10.15479/at:ista:9397">10.15479/at:ista:9397</a>.
  short: K. Huljev, Coordinated Spatiotemporal Reorganization of Interstitial Fluid
    Is Required for Axial Mesendoderm Migration in Zebrafish Gastrulation, Institute
    of Science and Technology Austria, 2021.
corr_author: '1'
date_created: 2021-05-17T12:31:30Z
date_published: 2021-05-18T00:00:00Z
date_updated: 2026-04-08T07:12:51Z
day: '18'
ddc:
- '571'
degree_awarded: PhD
department:
- _id: CaHe
- _id: GradSch
doi: 10.15479/at:ista:9397
file:
- access_level: closed
  checksum: 7f98532f5324a0b2f3fa8de2967baa19
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: khuljev
  date_created: 2021-05-17T12:29:12Z
  date_updated: 2022-05-21T22:30:04Z
  embargo_to: open_access
  file_id: '9398'
  file_name: KHuljev_Thesis_corrections.docx
  file_size: 47799741
  relation: source_file
- access_level: open_access
  checksum: bf512f8a1e572a543778fc4b227c01ba
  content_type: application/pdf
  creator: khuljev
  date_created: 2021-05-18T14:50:28Z
  date_updated: 2022-05-21T22:30:04Z
  embargo: 2022-05-20
  file_id: '9401'
  file_name: new_KHuljev_Thesis_corrections.pdf
  file_size: 16542131
  relation: main_file
file_date_updated: 2022-05-21T22:30:04Z
has_accepted_license: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: '101'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: Coordinated spatiotemporal reorganization of interstitial fluid is required
  for axial mesendoderm migration in zebrafish gastrulation
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2021'
...