---
res:
  bibo_abstract:
  - Microtubule plus-end depolymerization rate is a potentially important target of
    physiological regulation, but it has been challenging to measure, so its role
    in spatial organization is poorly understood. Here we apply a method for tracking
    plus ends based on time difference imaging to measure depolymerization rates in
    large interphase asters growing in Xenopus egg extract. We observed strong spatial
    regulation of depolymerization rates, which were higher in the aster interior
    compared with the periphery, and much less regulation of polymerization or catastrophe
    rates. We interpret these data in terms of a limiting component model, where aster
    growth results in lower levels of soluble tubulin and microtubule-associated proteins
    (MAPs) in the interior cytosol compared with that at the periphery. The steady-state
    polymer fraction of tubulin was ∼30%, so tubulin is not strongly depleted in the
    aster interior. We propose that the limiting component for microtubule assembly
    is a MAP that inhibits depolymerization, and that egg asters are tuned to low
    microtubule density.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Keisuke
      foaf_name: Ishihara, Keisuke
      foaf_surname: Ishihara
  - foaf_Person:
      foaf_givenName: Franziska
      foaf_name: Decker, Franziska
      foaf_surname: Decker
  - foaf_Person:
      foaf_givenName: Paulo R
      foaf_name: Dos Santos Caldas, Paulo R
      foaf_surname: Dos Santos Caldas
      foaf_workInfoHomepage: http://www.librecat.org/personId=38FCDB4C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6730-4461
  - foaf_Person:
      foaf_givenName: James F.
      foaf_name: Pelletier, James F.
      foaf_surname: Pelletier
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Loose, Martin
      foaf_surname: Loose
      foaf_workInfoHomepage: http://www.librecat.org/personId=462D4284-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7309-9724
  - foaf_Person:
      foaf_givenName: Jan
      foaf_name: Brugués, Jan
      foaf_surname: Brugués
  - foaf_Person:
      foaf_givenName: Timothy J.
      foaf_name: Mitchison, Timothy J.
      foaf_surname: Mitchison
  bibo_doi: 10.1091/MBC.E20-11-0723
  bibo_issue: '9'
  bibo_volume: 32
  dct_date: 2021^xs_gYear
  dct_identifier:
  - UT:000641574700005
  dct_isPartOf:
  - http://id.crossref.org/issn/1059-1524
  - http://id.crossref.org/issn/1939-4586
  dct_language: eng
  dct_publisher: American Society for Cell Biology@
  dct_title: Spatial variation of microtubule depolymerization in large asters@
  fabio_hasPubmedId: '33439671'
...