Decoding of position in the developing neural tube from antiparallel morphogen gradients

article Decoding of position in the developing neural tube from antiparallel morphogen gradients published yes Marcin P Zagórski author 343DA0DC-F248-11E8-B48F-1D18A9856A870000-0001-7896-7762 Yoji Tabata author Nathalie Brandenberg author Matthias Lutolf author Gasper Tkacik author 3D494DCA-F248-11E8-B48F-1D18A9856A870000-0002-6699-1455 Tobias Bollenbach author James Briscoe author Anna Kicheva author 3959A2A0-F248-11E8-B48F-1D18A9856A870000-0003-4509-4998 AnKi department GaTk department Biophysics of information processing in gene regulation project Coordination of Patterning And Growth In the Spinal Cord project International IST Postdoc Fellowship Programme project Developing High-Throughput Bioassays for Human Cancers in Zebrafish project Like many developing tissues, the vertebrate neural tube is patterned by antiparallel morphogen gradients. To understand how these inputs are interpreted, we measured morphogen signaling and target gene expression in mouse embryos and chick ex vivo assays. From these data, we derived and validated a characteristic decoding map that relates morphogen input to the positional identity of neural progenitors. Analysis of the observed responses indicates that the underlying interpretation strategy minimizes patterning errors in response to the joint input of noisy opposing gradients. We reverse-engineered a transcriptional network that provides a mechanistic basis for the observed cell fate decisions and accounts for the precision and dynamics of pattern formation. Together, our data link opposing gradient dynamics in a growing tissue to precise pattern formation. American Association for the Advancement of Science2017 eng Science 0036-8075 28663499 00040435150003610.1126/science.aam5887 35663451379 - 1383 Zagórski MP, Tabata Y, Brandenberg N, Lutolf M, Tkačik G, Bollenbach T, Briscoe J, Kicheva A. 2017. Decoding of position in the developing neural tube from antiparallel morphogen gradients. Science. 356(6345), 1379–1383. M.P. Zagórski, Y. Tabata, N. Brandenberg, M. Lutolf, G. Tkačik, T. Bollenbach, J. Briscoe, A. Kicheva, Science 356 (2017) 1379–1383. Zagórski, Marcin P., et al. “Decoding of Position in the Developing Neural Tube from Antiparallel Morphogen Gradients.” Science, vol. 356, no. 6345, American Association for the Advancement of Science, 2017, pp. 1379–83, doi:<a href="https://doi.org/10.1126/science.aam5887">10.1126/science.aam5887</a>. M. P. Zagórski et al., “Decoding of position in the developing neural tube from antiparallel morphogen gradients,” Science, vol. 356, no. 6345. American Association for the Advancement of Science, pp. 1379–1383, 2017. Zagórski, Marcin P, Yoji Tabata, Nathalie Brandenberg, Matthias Lutolf, Gašper Tkačik, Tobias Bollenbach, James Briscoe, and Anna Kicheva. “Decoding of Position in the Developing Neural Tube from Antiparallel Morphogen Gradients.” Science. American Association for the Advancement of Science, 2017. <a href="https://doi.org/10.1126/science.aam5887">https://doi.org/10.1126/science.aam5887</a>. Zagórski MP, Tabata Y, Brandenberg N, et al. Decoding of position in the developing neural tube from antiparallel morphogen gradients. Science. 2017;356(6345):1379-1383. doi:<a href="https://doi.org/10.1126/science.aam5887">10.1126/science.aam5887</a> Zagórski, M. P., Tabata, Y., Brandenberg, N., Lutolf, M., Tkačik, G., Bollenbach, T., … Kicheva, A. (2017). Decoding of position in the developing neural tube from antiparallel morphogen gradients. Science. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.aam5887">https://doi.org/10.1126/science.aam5887</a> 9432018-12-11T11:49:20Z2025-07-10T12:01:45Z