article published yes A. M. Lindroth author Xiaofeng Cao author James P. Jackson author Daniel Zilberman author 6973db13-dd5f-11ea-814e-b3e5455e9ed10000-0002-0123-8649 Claire M. McCallum author Steven Henikoff author Steven E. Jacobsen author DaZi department Epigenetic silenced alleles of the Arabidopsis SUPERMANlocus (the clark kent alleles) are associated with dense hypermethylation at noncanonical cytosines (CpXpG and asymmetric sites, where X = A, T, C, or G). A genetic screen for suppressors of a hypermethylated clark kent mutant identified nine loss-of-function alleles of CHROMOMETHYLASE3(CMT3), a novel cytosine methyltransferase homolog. These cmt3 mutants display a wild-type morphology but exhibit decreased CpXpG methylation of the SUP gene and of other sequences throughout the genome. They also show reactivated expression of endogenous retrotransposon sequences. These results show that a non-CpG DNA methyltransferase is responsible for maintaining epigenetic gene silencing. American Association for the Advancement of Science2001 eng Multidisciplinary 0036-8075 1095-9203 1134913810.1126/science.1059745 29255242077-2080 yes Lindroth, A. M., Xiaofeng Cao, James P. Jackson, Daniel Zilberman, Claire M. McCallum, Steven Henikoff, and Steven E. Jacobsen. “Requirement of CHROMOMETHYLASE3 for Maintenance of CpXpG Methylation.” <i>Science</i>. American Association for the Advancement of Science, 2001. <a href="https://doi.org/10.1126/science.1059745">https://doi.org/10.1126/science.1059745</a>. Lindroth, A. M., Cao, X., Jackson, J. P., Zilberman, D., McCallum, C. M., Henikoff, S., &#38; Jacobsen, S. E. (2001). Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation. <i>Science</i>. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.1059745">https://doi.org/10.1126/science.1059745</a> A.M. Lindroth, X. Cao, J.P. Jackson, D. Zilberman, C.M. McCallum, S. Henikoff, S.E. Jacobsen, Science 292 (2001) 2077–2080. Lindroth AM, Cao X, Jackson JP, Zilberman D, McCallum CM, Henikoff S, Jacobsen SE. 2001. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation. Science. 292(5524), 2077–2080. Lindroth, A. M., et al. “Requirement of CHROMOMETHYLASE3 for Maintenance of CpXpG Methylation.” <i>Science</i>, vol. 292, no. 5524, American Association for the Advancement of Science, 2001, pp. 2077–80, doi:<a href="https://doi.org/10.1126/science.1059745">10.1126/science.1059745</a>. A. M. Lindroth <i>et al.</i>, “Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation,” <i>Science</i>, vol. 292, no. 5524. American Association for the Advancement of Science, pp. 2077–2080, 2001. Lindroth AM, Cao X, Jackson JP, et al. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation. <i>Science</i>. 2001;292(5524):2077-2080. doi:<a href="https://doi.org/10.1126/science.1059745">10.1126/science.1059745</a> 94442021-06-02T13:35:16Z2021-12-14T08:40:32Z