---
_id: '9451'
abstract:
- lang: eng
  text: The Arabidopsis thaliana central cell, the companion cell of the egg, undergoes
    DNA demethylation before fertilization, but the targeting preferences, mechanism,
    and biological significance of this process remain unclear. Here, we show that
    active DNA demethylation mediated by the DEMETER DNA glycosylase accounts for
    all of the demethylation in the central cell and preferentially targets small,
    AT-rich, and nucleosome-depleted euchromatic transposable elements. The vegetative
    cell, the companion cell of sperm, also undergoes DEMETER-dependent demethylation
    of similar sequences, and lack of DEMETER in vegetative cells causes reduced small
    RNA–directed DNA methylation of transposons in sperm. Our results demonstrate
    that demethylation in companion cells reinforces transposon methylation in plant
    gametes and likely contributes to stable silencing of transposable elements across
    generations.
article_processing_charge: No
article_type: original
author:
- first_name: Christian A.
  full_name: Ibarra, Christian A.
  last_name: Ibarra
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  last_name: Feng
- first_name: Vera K.
  full_name: Schoft, Vera K.
  last_name: Schoft
- first_name: Tzung-Fu
  full_name: Hsieh, Tzung-Fu
  last_name: Hsieh
- first_name: Rie
  full_name: Uzawa, Rie
  last_name: Uzawa
- first_name: Jessica A.
  full_name: Rodrigues, Jessica A.
  last_name: Rodrigues
- first_name: Assaf
  full_name: Zemach, Assaf
  last_name: Zemach
- first_name: Nina
  full_name: Chumak, Nina
  last_name: Chumak
- first_name: Adriana
  full_name: Machlicova, Adriana
  last_name: Machlicova
- first_name: Toshiro
  full_name: Nishimura, Toshiro
  last_name: Nishimura
- first_name: Denisse
  full_name: Rojas, Denisse
  last_name: Rojas
- first_name: Robert L.
  full_name: Fischer, Robert L.
  last_name: Fischer
- first_name: Hisashi
  full_name: Tamaru, Hisashi
  last_name: Tamaru
- first_name: Daniel
  full_name: Zilberman, Daniel
  id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
  last_name: Zilberman
  orcid: 0000-0002-0123-8649
citation:
  ama: Ibarra CA, Feng X, Schoft VK, et al. Active DNA demethylation in plant companion
    cells reinforces transposon methylation in gametes. <i>Science</i>. 2012;337(6100):1360-1364.
    doi:<a href="https://doi.org/10.1126/science.1224839">10.1126/science.1224839</a>
  apa: Ibarra, C. A., Feng, X., Schoft, V. K., Hsieh, T.-F., Uzawa, R., Rodrigues,
    J. A., … Zilberman, D. (2012). Active DNA demethylation in plant companion cells
    reinforces transposon methylation in gametes. <i>Science</i>. American Association
    for the Advancement of Science. <a href="https://doi.org/10.1126/science.1224839">https://doi.org/10.1126/science.1224839</a>
  chicago: Ibarra, Christian A., Xiaoqi Feng, Vera K. Schoft, Tzung-Fu Hsieh, Rie
    Uzawa, Jessica A. Rodrigues, Assaf Zemach, et al. “Active DNA Demethylation in
    Plant Companion Cells Reinforces Transposon Methylation in Gametes.” <i>Science</i>.
    American Association for the Advancement of Science, 2012. <a href="https://doi.org/10.1126/science.1224839">https://doi.org/10.1126/science.1224839</a>.
  ieee: C. A. Ibarra <i>et al.</i>, “Active DNA demethylation in plant companion cells
    reinforces transposon methylation in gametes,” <i>Science</i>, vol. 337, no. 6100.
    American Association for the Advancement of Science, pp. 1360–1364, 2012.
  ista: Ibarra CA, Feng X, Schoft VK, Hsieh T-F, Uzawa R, Rodrigues JA, Zemach A,
    Chumak N, Machlicova A, Nishimura T, Rojas D, Fischer RL, Tamaru H, Zilberman
    D. 2012. Active DNA demethylation in plant companion cells reinforces transposon
    methylation in gametes. Science. 337(6100), 1360–1364.
  mla: Ibarra, Christian A., et al. “Active DNA Demethylation in Plant Companion Cells
    Reinforces Transposon Methylation in Gametes.” <i>Science</i>, vol. 337, no. 6100,
    American Association for the Advancement of Science, 2012, pp. 1360–64, doi:<a
    href="https://doi.org/10.1126/science.1224839">10.1126/science.1224839</a>.
  short: C.A. Ibarra, X. Feng, V.K. Schoft, T.-F. Hsieh, R. Uzawa, J.A. Rodrigues,
    A. Zemach, N. Chumak, A. Machlicova, T. Nishimura, D. Rojas, R.L. Fischer, H.
    Tamaru, D. Zilberman, Science 337 (2012) 1360–1364.
date_created: 2021-06-04T07:51:31Z
date_published: 2012-09-14T00:00:00Z
date_updated: 2021-12-14T08:28:51Z
day: '14'
ddc:
- '580'
department:
- _id: DaZi
doi: 10.1126/science.1224839
extern: '1'
external_id:
  pmid:
  - '22984074'
has_accepted_license: '1'
intvolume: '       337'
issue: '6100'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034762/
month: '09'
oa: 1
oa_version: Published Version
page: 1360-1364
pmid: 1
publication: Science
publication_identifier:
  eissn:
  - 1095-9203
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Active DNA demethylation in plant companion cells reinforces transposon methylation
  in gametes
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 337
year: '2012'
...