---
_id: '9473'
abstract:
- lang: eng
  text: Cytosine DNA methylation regulates the expression of eukaryotic genes and
    transposons. Methylation is copied by methyltransferases after DNA replication,
    which results in faithful transmission of methylation patterns during cell division
    and, at least in flowering plants, across generations. Transgenerational inheritance
    is mediated by a small group of cells that includes gametes and their progenitors.
    However, methylation is usually analyzed in somatic tissues that do not contribute
    to the next generation, and the mechanisms of transgenerational inheritance are
    inferred from such studies. To gain a better understanding of how DNA methylation
    is inherited, we analyzed purified Arabidopsis thaliana sperm and vegetative cells-the
    cell types that comprise pollen-with mutations in the DRM, CMT2, and CMT3 methyltransferases.
    We find that DNA methylation dependency on these enzymes is similar in sperm,
    vegetative cells, and somatic tissues, although DRM activity extends into heterochromatin
    in vegetative cells, likely reflecting transcription of heterochromatic transposons
    in this cell type. We also show that lack of histone H1, which elevates heterochromatic
    DNA methylation in somatic tissues, does not have this effect in pollen. Instead,
    levels of CG methylation in wild-type sperm and vegetative cells, as well as in
    wild-type microspores from which both pollen cell types originate, are substantially
    higher than in wild-type somatic tissues and similar to those of H1-depleted roots.
    Our results demonstrate that the mechanisms of methylation maintenance are similar
    between pollen and somatic cells, but the efficiency of CG methylation is higher
    in pollen, allowing methylation patterns to be accurately inherited across generations.
article_processing_charge: No
article_type: original
author:
- first_name: Ping-Hung
  full_name: Hsieh, Ping-Hung
  last_name: Hsieh
- first_name: Shengbo
  full_name: He, Shengbo
  last_name: He
- first_name: Toby
  full_name: Buttress, Toby
  last_name: Buttress
- first_name: Hongbo
  full_name: Gao, Hongbo
  last_name: Gao
- first_name: Matthew
  full_name: Couchman, Matthew
  last_name: Couchman
- first_name: Robert L.
  full_name: Fischer, Robert L.
  last_name: Fischer
- first_name: Daniel
  full_name: Zilberman, Daniel
  id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
  last_name: Zilberman
  orcid: 0000-0002-0123-8649
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
citation:
  ama: Hsieh P-H, He S, Buttress T, et al. Arabidopsis male sexual lineage exhibits
    more robust maintenance of CG methylation than somatic tissues. <i>Proceedings
    of the National Academy of Sciences</i>. 2016;113(52):15132-15137. doi:<a href="https://doi.org/10.1073/pnas.1619074114">10.1073/pnas.1619074114</a>
  apa: Hsieh, P.-H., He, S., Buttress, T., Gao, H., Couchman, M., Fischer, R. L.,
    … Feng, X. (2016). Arabidopsis male sexual lineage exhibits more robust maintenance
    of CG methylation than somatic tissues. <i>Proceedings of the National Academy
    of Sciences</i>. National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.1619074114">https://doi.org/10.1073/pnas.1619074114</a>
  chicago: Hsieh, Ping-Hung, Shengbo He, Toby Buttress, Hongbo Gao, Matthew Couchman,
    Robert L. Fischer, Daniel Zilberman, and Xiaoqi Feng. “Arabidopsis Male Sexual
    Lineage Exhibits More Robust Maintenance of CG Methylation than Somatic Tissues.”
    <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences,
    2016. <a href="https://doi.org/10.1073/pnas.1619074114">https://doi.org/10.1073/pnas.1619074114</a>.
  ieee: P.-H. Hsieh <i>et al.</i>, “Arabidopsis male sexual lineage exhibits more
    robust maintenance of CG methylation than somatic tissues,” <i>Proceedings of
    the National Academy of Sciences</i>, vol. 113, no. 52. National Academy of Sciences,
    pp. 15132–15137, 2016.
  ista: Hsieh P-H, He S, Buttress T, Gao H, Couchman M, Fischer RL, Zilberman D, Feng
    X. 2016. Arabidopsis male sexual lineage exhibits more robust maintenance of CG
    methylation than somatic tissues. Proceedings of the National Academy of Sciences.
    113(52), 15132–15137.
  mla: Hsieh, Ping-Hung, et al. “Arabidopsis Male Sexual Lineage Exhibits More Robust
    Maintenance of CG Methylation than Somatic Tissues.” <i>Proceedings of the National
    Academy of Sciences</i>, vol. 113, no. 52, National Academy of Sciences, 2016,
    pp. 15132–37, doi:<a href="https://doi.org/10.1073/pnas.1619074114">10.1073/pnas.1619074114</a>.
  short: P.-H. Hsieh, S. He, T. Buttress, H. Gao, M. Couchman, R.L. Fischer, D. Zilberman,
    X. Feng, Proceedings of the National Academy of Sciences 113 (2016) 15132–15137.
date_created: 2021-06-07T06:21:39Z
date_published: 2016-12-27T00:00:00Z
date_updated: 2023-05-08T11:00:40Z
day: '27'
department:
- _id: DaZi
- _id: XiFe
doi: 10.1073/pnas.1619074114
extern: '1'
external_id:
  pmid:
  - '27956643'
intvolume: '       113'
issue: '52'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1073/pnas.1619074114
month: '12'
oa: 1
oa_version: Published Version
page: 15132-15137
pmid: 1
publication: Proceedings of the National Academy of Sciences
publication_identifier:
  eissn:
  - 1091-6490
  issn:
  - 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation
  than somatic tissues
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...