---
res:
  bibo_abstract:
  - Multicellular eukaryotes produce small RNA molecules (approximately 21–24 nucleotides)
    of two general types, microRNA (miRNA) and short interfering RNA (siRNA). They
    collectively function as sequence-specific guides to silence or regulate genes,
    transposons, and viruses and to modify chromatin and genome structure. Formation
    or activity of small RNAs requires factors belonging to gene families that encode
    DICER (or DICER-LIKE [DCL]) and ARGONAUTE proteins and, in the case of some siRNAs,
    RNA-dependent RNA polymerase (RDR) proteins. Unlike many animals, plants encode
    multiple DCL and RDR proteins. Using a series of insertion mutants of Arabidopsis
    thaliana, unique functions for three DCL proteins in miRNA (DCL1), endogenous
    siRNA (DCL3), and viral siRNA (DCL2) biogenesis were identified. One RDR protein
    (RDR2) was required for all endogenous siRNAs analyzed. The loss of endogenous
    siRNA in dcl3 and rdr2 mutants was associated with loss of heterochromatic marks
    and increased transcript accumulation at some loci. Defects in siRNA-generation
    activity in response to turnip crinkle virus in dcl2 mutant plants correlated
    with increased virus susceptibility. We conclude that proliferation and diversification
    of DCL and RDR genes during evolution of plants contributed to specialization
    of small RNA-directed pathways for development, chromatin structure, and defense.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Zhixin
      foaf_name: Xie, Zhixin
      foaf_surname: Xie
  - foaf_Person:
      foaf_givenName: Lisa K.
      foaf_name: Johansen, Lisa K.
      foaf_surname: Johansen
  - foaf_Person:
      foaf_givenName: Adam M.
      foaf_name: Gustafson, Adam M.
      foaf_surname: Gustafson
  - foaf_Person:
      foaf_givenName: Kristin D.
      foaf_name: Kasschau, Kristin D.
      foaf_surname: Kasschau
  - foaf_Person:
      foaf_givenName: 'Andrew D. '
      foaf_name: 'Lellis, Andrew D. '
      foaf_surname: Lellis
  - foaf_Person:
      foaf_givenName: Daniel
      foaf_name: Zilberman, Daniel
      foaf_surname: Zilberman
      foaf_workInfoHomepage: http://www.librecat.org/personId=6973db13-dd5f-11ea-814e-b3e5455e9ed1
    orcid: 0000-0002-0123-8649
  - foaf_Person:
      foaf_givenName: Steven E.
      foaf_name: Jacobsen, Steven E.
      foaf_surname: Jacobsen
  - foaf_Person:
      foaf_givenName: James C.
      foaf_name: Carrington, James C.
      foaf_surname: Carrington
  bibo_doi: 10.1371/journal.pbio.0020104
  bibo_issue: '5'
  bibo_volume: 2
  dct_date: 2004^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1544-9173
  - http://id.crossref.org/issn/1545-7885
  dct_language: eng
  dct_publisher: Public Library of Science@
  dct_title: Genetic and functional diversification of small RNA pathways in plants@
  fabio_hasPubmedId: '15024409'
...