---
OA_place: publisher
OA_type: hybrid
_id: '9526'
abstract:
- lang: eng
  text: DNA methylation and histone H1 mediate transcriptional silencing of genes
    and transposable elements, but how they interact is unclear. In plants and animals
    with mosaic genomic methylation, functionally mysterious methylation is also common
    within constitutively active housekeeping genes. Here, we show that H1 is enriched
    in methylated sequences, including genes, of Arabidopsis thaliana, yet this enrichment
    is independent of DNA methylation. Loss of H1 disperses heterochromatin, globally
    alters nucleosome organization, and activates H1-bound genes, but only weakly
    de-represses transposable elements. However, H1 loss strongly activates transposable
    elements hypomethylated through mutation of DNA methyltransferase MET1. Hypomethylation
    of genes also activates antisense transcription, which is modestly enhanced by
    H1 loss. Our results demonstrate that H1 and DNA methylation jointly maintain
    transcriptional homeostasis by silencing transposable elements and aberrant intragenic
    transcripts. Such functionality plausibly explains why DNA methylation, a well-known
    mutagen, has been maintained within coding sequences of crucial plant and animal
    genes.
article_processing_charge: No
article_type: original
author:
- first_name: Jaemyung
  full_name: Choi, Jaemyung
  last_name: Choi
- first_name: David B.
  full_name: Lyons, David B.
  last_name: Lyons
- first_name: M. Yvonne
  full_name: Kim, M. Yvonne
  last_name: Kim
- first_name: Jonathan D.
  full_name: Moore, Jonathan D.
  last_name: Moore
- first_name: Daniel
  full_name: Zilberman, Daniel
  id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
  last_name: Zilberman
  orcid: 0000-0002-0123-8649
citation:
  ama: Choi J, Lyons DB, Kim MY, Moore JD, Zilberman D. DNA methylation and histone
    H1 jointly repress transposable elements and aberrant intragenic transcripts.
    <i>Molecular Cell</i>. 2020;77(2):310-323.e7. doi:<a href="https://doi.org/10.1016/j.molcel.2019.10.011">10.1016/j.molcel.2019.10.011</a>
  apa: Choi, J., Lyons, D. B., Kim, M. Y., Moore, J. D., &#38; Zilberman, D. (2020).
    DNA methylation and histone H1 jointly repress transposable elements and aberrant
    intragenic transcripts. <i>Molecular Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.molcel.2019.10.011">https://doi.org/10.1016/j.molcel.2019.10.011</a>
  chicago: Choi, Jaemyung, David B. Lyons, M. Yvonne Kim, Jonathan D. Moore, and Daniel
    Zilberman. “DNA Methylation and Histone H1 Jointly Repress Transposable Elements
    and Aberrant Intragenic Transcripts.” <i>Molecular Cell</i>. Elsevier, 2020. <a
    href="https://doi.org/10.1016/j.molcel.2019.10.011">https://doi.org/10.1016/j.molcel.2019.10.011</a>.
  ieee: J. Choi, D. B. Lyons, M. Y. Kim, J. D. Moore, and D. Zilberman, “DNA methylation
    and histone H1 jointly repress transposable elements and aberrant intragenic transcripts,”
    <i>Molecular Cell</i>, vol. 77, no. 2. Elsevier, p. 310–323.e7, 2020.
  ista: Choi J, Lyons DB, Kim MY, Moore JD, Zilberman D. 2020. DNA methylation and
    histone H1 jointly repress transposable elements and aberrant intragenic transcripts.
    Molecular Cell. 77(2), 310–323.e7.
  mla: Choi, Jaemyung, et al. “DNA Methylation and Histone H1 Jointly Repress Transposable
    Elements and Aberrant Intragenic Transcripts.” <i>Molecular Cell</i>, vol. 77,
    no. 2, Elsevier, 2020, p. 310–323.e7, doi:<a href="https://doi.org/10.1016/j.molcel.2019.10.011">10.1016/j.molcel.2019.10.011</a>.
  short: J. Choi, D.B. Lyons, M.Y. Kim, J.D. Moore, D. Zilberman, Molecular Cell 77
    (2020) 310–323.e7.
date_created: 2021-06-08T06:37:09Z
date_published: 2020-01-16T00:00:00Z
date_updated: 2024-10-16T12:14:37Z
day: '16'
department:
- _id: DaZi
doi: 10.1016/j.molcel.2019.10.011
extern: '1'
external_id:
  pmid:
  - '31732458'
intvolume: '        77'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.molcel.2019.10.011
month: '01'
oa: 1
oa_version: Published Version
page: 310-323.e7
pmid: 1
publication: Molecular Cell
publication_identifier:
  eissn:
  - 1097-4164
  issn:
  - 1097-2765
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: DNA methylation and histone H1 jointly repress transposable elements and aberrant
  intragenic transcripts
type: journal_article
user_id: 0043cee0-e5fc-11ee-9736-f83bc23afbf0
volume: 77
year: '2020'
...