---
res:
  bibo_abstract:
  - Left-right asymmetries can be considered a fundamental organizational principle
    of the vertebrate central nervous system. The hippocampal CA3-CA1 pyramidal cell
    synaptic connection shows an input-side dependent asymmetry where the hemispheric
    location of the presynaptic CA3 neuron determines the synaptic properties. Left-input
    synapses terminating on apical dendrites in stratum radiatum have a higher density
    of NMDA receptor subunit GluN2B, a lower density of AMPA receptor subunit GluA1
    and smaller areas with less often perforated PSDs. On the other hand, left-input
    synapses terminating on basal dendrites in stratum oriens have lower GluN2B densities
    than right-input ones. Apical and basal synapses further employ different signaling
    pathways involved in LTP. SDS-digested freeze-fracture replica labeling can visualize
    synaptic membrane proteins with high sensitivity and resolution, and has been
    used to reveal the asymmetry at the electron microscopic level. However, it requires
    time-consuming manual demarcation of the synaptic surface for quantitative measurements.
    To facilitate the analysis of replica labeling, I first developed a software named
    Darea, which utilizes deep-learning to automatize this demarcation. With Darea
    I characterized the synaptic distribution of NMDA and AMPA receptors as well as
    the voltage-gated Ca2+ channels in CA1 stratum radiatum and oriens. Second, I
    explored the role of GluN2B and its carboxy-terminus in the establishment of input-side
    dependent hippocampal asymmetry. In conditional knock-out mice lacking GluN2B
    expression in CA1 and GluN2B-2A swap mice, where GluN2B carboxy-terminus was exchanged
    to that of GluN2A, no significant asymmetries of GluN2B, GluA1 and PSD area were
    detected. We further discovered a previously unknown functional asymmetry of GluN2A,
    which was also lost in the swap mouse. These results demonstrate that GluN2B carboxy-terminus
    plays a critical role in normal formation of input-side dependent asymmetry.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: David
      foaf_name: Kleindienst, David
      foaf_surname: Kleindienst
      foaf_workInfoHomepage: http://www.librecat.org/personId=42E121A4-F248-11E8-B48F-1D18A9856A87
  bibo_doi: 10.15479/at:ista:9562
  dct_date: 2021^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2663-337X
  dct_language: eng
  dct_publisher: Institute of Science and Technology Austria@
  dct_title: '2B or not 2B: Hippocampal asymmetries mediated by NMDA receptor subunit
    GluN2B C-terminus and high-throughput image analysis by Deep-Learning@'
...