---
res:
  bibo_abstract:
  - Biosensors that exploit Forster resonance energy transfer (FRET) can be used to
    visualize biological and physiological processes and are capable of providing
    detailed information in both spatial and temporal dimensions. In a FRET-based
    biosensor, substrate binding is associated with a change in the relative positions
    of two fluorophores, leading to a change in FRET efficiency that may be observed
    in the fluorescence spectrum. As a result, their design requires a ligand-binding
    protein that exhibits a conformational change upon binding. However, not all ligand-binding
    proteins produce responsive sensors upon conjugation to fluorescent proteins or
    dyes, and identifying the optimum locations for the fluorophores often involves
    labor-intensive iterative design or high-throughput screening. Combining the genetic
    fusion of a fluorescent protein to the ligand-binding protein with site-specific
    covalent attachment of a fluorescent dye can allow fine control over the positions
    of the two fluorophores, allowing the construction of very sensitive sensors.
    This relies upon the accurate prediction of the locations of the two fluorophores
    in bound and unbound states. In this chapter, we describe a method for computational
    identification of dye-attachment sites that allows the use of cysteine modification
    to attach synthetic dyes that can be paired with a fluorescent protein for the
    purposes of creating FRET sensors.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Joshua
      foaf_name: Mitchell, Joshua
      foaf_surname: Mitchell
  - foaf_Person:
      foaf_givenName: William
      foaf_name: Zhang, William
      foaf_surname: Zhang
  - foaf_Person:
      foaf_givenName: Michel
      foaf_name: Herde, Michel
      foaf_surname: Herde
  - foaf_Person:
      foaf_givenName: Christian
      foaf_name: Henneberger, Christian
      foaf_surname: Henneberger
  - foaf_Person:
      foaf_givenName: Harald L
      foaf_name: Janovjak, Harald L
      foaf_surname: Janovjak
      foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8023-9315
  - foaf_Person:
      foaf_givenName: Megan
      foaf_name: O'Mara, Megan
      foaf_surname: O'Mara
  - foaf_Person:
      foaf_givenName: Colin
      foaf_name: Jackson, Colin
      foaf_surname: Jackson
  bibo_doi: 10.1007/978-1-4939-6940-1_6
  bibo_volume: 1596
  dct_date: 2017^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1064-3745
  dct_language: eng
  dct_publisher: Springer@
  dct_title: Method for developing optical sensors using a synthetic dye fluorescent
    protein FRET pair and computational modeling and assessment@
...