---
_id: '9601'
abstract:
- lang: eng
  text: 'In mammalian genomes, differentially methylated regions (DMRs) and histone
    marks including trimethylation of histone 3 lysine 27 (H3K27me3) at imprinted
    genes are asymmetrically inherited to control parentally-biased gene expression.
    However, neither parent-of-origin-specific transcription nor imprints have been
    comprehensively mapped at the blastocyst stage of preimplantation development.
    Here, we address this by integrating transcriptomic and epigenomic approaches
    in mouse preimplantation embryos. We find that seventy-one genes exhibit previously
    unreported parent-of-origin-specific expression in blastocysts (nBiX: novel blastocyst-imprinted
    expressed). Uniparental expression of nBiX genes disappears soon after implantation.
    Micro-whole-genome bisulfite sequencing (µWGBS) of individual uniparental blastocysts
    detects 859 DMRs. We further find that 16% of nBiX genes are associated with a
    DMR, whereas most are associated with parentally-biased H3K27me3, suggesting a
    role for Polycomb-mediated imprinting in blastocysts. nBiX genes are clustered:
    five clusters contained at least one published imprinted gene, and five clusters
    exclusively contained nBiX genes. These data suggest that early development undergoes
    a complex program of stage-specific imprinting involving different tiers of regulation.'
acknowledgement: The authors thank Robert Feil and Anton Wutz for helpful discussions
  and comments, Samuel Collombet and Peter Fraser for sharing embryo TAD coordinates,
  and Andy Riddel at the Cambridge Stem Cell Institute and Thomas Sauer at the Max
  Perutz Laboratories FACS facility for flow-sorting. We thank the team of the Biomedical
  Sequencing Facility at the CeMM and the Vienna Biocenter Core Facilities (VBCF)
  for support with next-generation sequencing. We are grateful to animal care teams
  at the University of Bath and MRC Harwell. A.C.F.P. acknowledges support from the
  UK Medical Research Council (MR/N000080/1 and MR/N020294/1) and Biotechnology and
  Biological Sciences Research Council (BB/P009506/1). L.S. is part of the FWF doctoral
  programme SMICH and supported by an Austrian Academy of Sciences DOC Fellowship.
  M.L. is funded by a Vienna Research Group for Young Investigators grant (VRG14-006)
  by the Vienna Science and Technology Fund (WWTF) and by the Austrian Science Fund
  FWF (I3786 and P31334).
article_number: '3804'
article_processing_charge: No
article_type: original
author:
- first_name: Laura
  full_name: Santini, Laura
  last_name: Santini
- first_name: Florian
  full_name: Halbritter, Florian
  last_name: Halbritter
- first_name: Fabian
  full_name: Titz-Teixeira, Fabian
  last_name: Titz-Teixeira
- first_name: Toru
  full_name: Suzuki, Toru
  last_name: Suzuki
- first_name: Maki
  full_name: Asami, Maki
  last_name: Asami
- first_name: Xiaoyan
  full_name: Ma, Xiaoyan
  last_name: Ma
- first_name: Julia
  full_name: Ramesmayer, Julia
  last_name: Ramesmayer
- first_name: Andreas
  full_name: Lackner, Andreas
  last_name: Lackner
- first_name: Nick
  full_name: Warr, Nick
  last_name: Warr
- first_name: Florian
  full_name: Pauler, Florian
  id: 48EA0138-F248-11E8-B48F-1D18A9856A87
  last_name: Pauler
  orcid: 0000-0002-7462-0048
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Ernest
  full_name: Laue, Ernest
  last_name: Laue
- first_name: Matthias
  full_name: Farlik, Matthias
  last_name: Farlik
- first_name: Christoph
  full_name: Bock, Christoph
  last_name: Bock
- first_name: Andreas
  full_name: Beyer, Andreas
  last_name: Beyer
- first_name: Anthony C.F.
  full_name: Perry, Anthony C.F.
  last_name: Perry
- first_name: Martin
  full_name: Leeb, Martin
  last_name: Leeb
citation:
  ama: Santini L, Halbritter F, Titz-Teixeira F, et al. Genomic imprinting in mouse
    blastocysts is predominantly associated with H3K27me3. <i>Nature Communications</i>.
    2021;12(1). doi:<a href="https://doi.org/10.1038/s41467-021-23510-4">10.1038/s41467-021-23510-4</a>
  apa: Santini, L., Halbritter, F., Titz-Teixeira, F., Suzuki, T., Asami, M., Ma,
    X., … Leeb, M. (2021). Genomic imprinting in mouse blastocysts is predominantly
    associated with H3K27me3. <i>Nature Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-021-23510-4">https://doi.org/10.1038/s41467-021-23510-4</a>
  chicago: Santini, Laura, Florian Halbritter, Fabian Titz-Teixeira, Toru Suzuki,
    Maki Asami, Xiaoyan Ma, Julia Ramesmayer, et al. “Genomic Imprinting in Mouse
    Blastocysts Is Predominantly Associated with H3K27me3.” <i>Nature Communications</i>.
    Springer Nature, 2021. <a href="https://doi.org/10.1038/s41467-021-23510-4">https://doi.org/10.1038/s41467-021-23510-4</a>.
  ieee: L. Santini <i>et al.</i>, “Genomic imprinting in mouse blastocysts is predominantly
    associated with H3K27me3,” <i>Nature Communications</i>, vol. 12, no. 1. Springer
    Nature, 2021.
  ista: Santini L, Halbritter F, Titz-Teixeira F, Suzuki T, Asami M, Ma X, Ramesmayer
    J, Lackner A, Warr N, Pauler F, Hippenmeyer S, Laue E, Farlik M, Bock C, Beyer
    A, Perry ACF, Leeb M. 2021. Genomic imprinting in mouse blastocysts is predominantly
    associated with H3K27me3. Nature Communications. 12(1), 3804.
  mla: Santini, Laura, et al. “Genomic Imprinting in Mouse Blastocysts Is Predominantly
    Associated with H3K27me3.” <i>Nature Communications</i>, vol. 12, no. 1, 3804,
    Springer Nature, 2021, doi:<a href="https://doi.org/10.1038/s41467-021-23510-4">10.1038/s41467-021-23510-4</a>.
  short: L. Santini, F. Halbritter, F. Titz-Teixeira, T. Suzuki, M. Asami, X. Ma,
    J. Ramesmayer, A. Lackner, N. Warr, F. Pauler, S. Hippenmeyer, E. Laue, M. Farlik,
    C. Bock, A. Beyer, A.C.F. Perry, M. Leeb, Nature Communications 12 (2021).
date_created: 2021-06-27T22:01:46Z
date_published: 2021-07-12T00:00:00Z
date_updated: 2026-04-02T13:55:23Z
day: '12'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1038/s41467-021-23510-4
external_id:
  isi:
  - '000667248600005'
file:
- access_level: open_access
  checksum: 75dd89d09945185b2d14b2434a0bcb50
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-06-28T08:04:22Z
  date_updated: 2021-06-28T08:04:22Z
  file_id: '9608'
  file_name: 2021_NatureCommunications_Santini.pdf
  file_size: 2156554
  relation: main_file
  success: 1
file_date_updated: 2021-06-28T08:04:22Z
has_accepted_license: '1'
intvolume: '        12'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '07'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
  eissn:
  - 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genomic imprinting in mouse blastocysts is predominantly associated with H3K27me3
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 12
year: '2021'
...