---
res:
  bibo_abstract:
  - Correlative microscopy incorporates the specificity of fluorescent protein labeling
    into high-resolution electron micrographs. Several approaches exist for correlative
    microscopy, most of which have used the green fluorescent protein (GFP) as the
    label for light microscopy. Here we use chemical tagging and synthetic fluorophores
    instead, in order to achieve protein-specific labeling, and to perform multicolor
    imaging. We show that synthetic fluorophores preserve their post-embedding fluorescence
    in the presence of uranyl acetate. Post-embedding fluorescence is of such quality
    that the specimen can be prepared with identical protocols for scanning electron
    microscopy (SEM) and transmission electron microscopy (TEM); this is particularly
    valuable when singular or otherwise difficult samples are examined. We show that
    synthetic fluorophores give bright, well-resolved signals in super-resolution
    light microscopy, enabling us to superimpose light microscopic images with a precision
    of up to 25 nm in the x–y plane on electron micrographs. To exemplify the preservation
    quality of our new method we visualize the molecular arrangement of cadherins
    in adherens junctions of mouse epithelial cells.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Mario
      foaf_name: Perkovic, Mario
      foaf_surname: Perkovic
  - foaf_Person:
      foaf_givenName: Michael
      foaf_name: Kunz, Michael
      foaf_surname: Kunz
  - foaf_Person:
      foaf_givenName: Ulrike
      foaf_name: Endesfelder, Ulrike
      foaf_surname: Endesfelder
  - foaf_Person:
      foaf_givenName: Stefanie
      foaf_name: Bunse, Stefanie
      foaf_surname: Bunse
  - foaf_Person:
      foaf_givenName: Christoph
      foaf_name: Wigge, Christoph
      foaf_surname: Wigge
  - foaf_Person:
      foaf_givenName: Zhou
      foaf_name: Yu, Zhou
      foaf_surname: Yu
  - foaf_Person:
      foaf_givenName: Victor-Valentin
      foaf_name: Hodirnau, Victor-Valentin
      foaf_surname: Hodirnau
      foaf_workInfoHomepage: http://www.librecat.org/personId=3661B498-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Margot P.
      foaf_name: Scheffer, Margot P.
      foaf_surname: Scheffer
  - foaf_Person:
      foaf_givenName: Anja
      foaf_name: Seybert, Anja
      foaf_surname: Seybert
  - foaf_Person:
      foaf_givenName: Sebastian
      foaf_name: Malkusch, Sebastian
      foaf_surname: Malkusch
  - foaf_Person:
      foaf_givenName: Erin M.
      foaf_name: Schuman, Erin M.
      foaf_surname: Schuman
  - foaf_Person:
      foaf_givenName: Mike
      foaf_name: Heilemann, Mike
      foaf_surname: Heilemann
  - foaf_Person:
      foaf_givenName: Achilleas S.
      foaf_name: Frangakis, Achilleas S.
      foaf_surname: Frangakis
  bibo_doi: 10.1016/j.jsb.2014.03.018
  bibo_issue: '2'
  bibo_volume: 186
  dct_date: 2014^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1047-8477
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Correlative light- and electron microscopy with chemical tags@
  fabio_hasPubmedId: '24698954'
...