---
_id: '9655'
abstract:
- lang: eng
  text: Correlative microscopy incorporates the specificity of fluorescent protein
    labeling into high-resolution electron micrographs. Several approaches exist for
    correlative microscopy, most of which have used the green fluorescent protein
    (GFP) as the label for light microscopy. Here we use chemical tagging and synthetic
    fluorophores instead, in order to achieve protein-specific labeling, and to perform
    multicolor imaging. We show that synthetic fluorophores preserve their post-embedding
    fluorescence in the presence of uranyl acetate. Post-embedding fluorescence is
    of such quality that the specimen can be prepared with identical protocols for
    scanning electron microscopy (SEM) and transmission electron microscopy (TEM);
    this is particularly valuable when singular or otherwise difficult samples are
    examined. We show that synthetic fluorophores give bright, well-resolved signals
    in super-resolution light microscopy, enabling us to superimpose light microscopic
    images with a precision of up to 25 nm in the x–y plane on electron micrographs.
    To exemplify the preservation quality of our new method we visualize the molecular
    arrangement of cadherins in adherens junctions of mouse epithelial cells.
article_processing_charge: No
article_type: original
author:
- first_name: Mario
  full_name: Perkovic, Mario
  last_name: Perkovic
- first_name: Michael
  full_name: Kunz, Michael
  last_name: Kunz
- first_name: Ulrike
  full_name: Endesfelder, Ulrike
  last_name: Endesfelder
- first_name: Stefanie
  full_name: Bunse, Stefanie
  last_name: Bunse
- first_name: Christoph
  full_name: Wigge, Christoph
  last_name: Wigge
- first_name: Zhou
  full_name: Yu, Zhou
  last_name: Yu
- first_name: Victor-Valentin
  full_name: Hodirnau, Victor-Valentin
  id: 3661B498-F248-11E8-B48F-1D18A9856A87
  last_name: Hodirnau
- first_name: Margot P.
  full_name: Scheffer, Margot P.
  last_name: Scheffer
- first_name: Anja
  full_name: Seybert, Anja
  last_name: Seybert
- first_name: Sebastian
  full_name: Malkusch, Sebastian
  last_name: Malkusch
- first_name: Erin M.
  full_name: Schuman, Erin M.
  last_name: Schuman
- first_name: Mike
  full_name: Heilemann, Mike
  last_name: Heilemann
- first_name: Achilleas S.
  full_name: Frangakis, Achilleas S.
  last_name: Frangakis
citation:
  ama: Perkovic M, Kunz M, Endesfelder U, et al. Correlative light- and electron microscopy
    with chemical tags. <i>Journal of Structural Biology</i>. 2014;186(2):205-213.
    doi:<a href="https://doi.org/10.1016/j.jsb.2014.03.018">10.1016/j.jsb.2014.03.018</a>
  apa: Perkovic, M., Kunz, M., Endesfelder, U., Bunse, S., Wigge, C., Yu, Z., … Frangakis,
    A. S. (2014). Correlative light- and electron microscopy with chemical tags. <i>Journal
    of Structural Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.jsb.2014.03.018">https://doi.org/10.1016/j.jsb.2014.03.018</a>
  chicago: Perkovic, Mario, Michael Kunz, Ulrike Endesfelder, Stefanie Bunse, Christoph
    Wigge, Zhou Yu, Victor-Valentin Hodirnau, et al. “Correlative Light- and Electron
    Microscopy with Chemical Tags.” <i>Journal of Structural Biology</i>. Elsevier,
    2014. <a href="https://doi.org/10.1016/j.jsb.2014.03.018">https://doi.org/10.1016/j.jsb.2014.03.018</a>.
  ieee: M. Perkovic <i>et al.</i>, “Correlative light- and electron microscopy with
    chemical tags,” <i>Journal of Structural Biology</i>, vol. 186, no. 2. Elsevier,
    pp. 205–213, 2014.
  ista: Perkovic M, Kunz M, Endesfelder U, Bunse S, Wigge C, Yu Z, Hodirnau V-V, Scheffer
    MP, Seybert A, Malkusch S, Schuman EM, Heilemann M, Frangakis AS. 2014. Correlative
    light- and electron microscopy with chemical tags. Journal of Structural Biology.
    186(2), 205–213.
  mla: Perkovic, Mario, et al. “Correlative Light- and Electron Microscopy with Chemical
    Tags.” <i>Journal of Structural Biology</i>, vol. 186, no. 2, Elsevier, 2014,
    pp. 205–13, doi:<a href="https://doi.org/10.1016/j.jsb.2014.03.018">10.1016/j.jsb.2014.03.018</a>.
  short: M. Perkovic, M. Kunz, U. Endesfelder, S. Bunse, C. Wigge, Z. Yu, V.-V. Hodirnau,
    M.P. Scheffer, A. Seybert, S. Malkusch, E.M. Schuman, M. Heilemann, A.S. Frangakis,
    Journal of Structural Biology 186 (2014) 205–213.
date_created: 2021-07-14T09:05:42Z
date_published: 2014-05-01T00:00:00Z
date_updated: 2021-07-22T08:26:32Z
day: '01'
ddc:
- '570'
doi: 10.1016/j.jsb.2014.03.018
extern: '1'
external_id:
  pmid:
  - '24698954'
file:
- access_level: open_access
  checksum: a322991b43cdc5935c99db88d285aa3a
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-07-22T08:06:34Z
  date_updated: 2021-07-22T08:06:34Z
  file_id: '9701'
  file_name: 2014_JournalOfStructuralBiology_Perkovic.pdf
  file_size: 3454628
  relation: main_file
  success: 1
file_date_updated: 2021-07-22T08:06:34Z
has_accepted_license: '1'
intvolume: '       186'
issue: '2'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/3.0/
month: '05'
oa: 1
oa_version: Published Version
page: 205-213
pmid: 1
publication: Journal of Structural Biology
publication_identifier:
  issn:
  - 1047-8477
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Correlative light- and electron microscopy with chemical tags
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/3.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND
    3.0)
  short: CC BY-NC-ND (3.0)
type: journal_article
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
volume: 186
year: '2014'
...