--- res: bibo_abstract: - Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase, and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension stabilizing E-cadherin-actin complexes at the contact.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jana foaf_name: Slovakova, Jana foaf_surname: Slovakova foaf_workInfoHomepage: http://www.librecat.org/personId=30F3F2F0-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Mateusz K foaf_name: Sikora, Mateusz K foaf_surname: Sikora foaf_workInfoHomepage: http://www.librecat.org/personId=2F74BCDE-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Silvia foaf_name: Caballero Mancebo, Silvia foaf_surname: Caballero Mancebo foaf_workInfoHomepage: http://www.librecat.org/personId=2F1E1758-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-5223-3346 - foaf_Person: foaf_givenName: Gabriel foaf_name: Krens, Gabriel foaf_surname: Krens foaf_workInfoHomepage: http://www.librecat.org/personId=2B819732-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4761-5996 - foaf_Person: foaf_givenName: Walter foaf_name: Kaufmann, Walter foaf_surname: Kaufmann foaf_workInfoHomepage: http://www.librecat.org/personId=3F99E422-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9735-5315 - foaf_Person: foaf_givenName: Karla foaf_name: Huljev, Karla foaf_surname: Huljev foaf_workInfoHomepage: http://www.librecat.org/personId=44C6F6A6-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Carl-Philipp J foaf_name: Heisenberg, Carl-Philipp J foaf_surname: Heisenberg foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0912-4566 bibo_doi: 10.1101/2020.11.20.391284 dct_date: 2020^xs_gYear dct_language: eng dct_publisher: Cold Spring Harbor Laboratory@ dct_title: Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion@ ...