Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion

preprint Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion published Jana Slovakova author 30F3F2F0-F248-11E8-B48F-1D18A9856A87 Mateusz K Sikora author 2F74BCDE-F248-11E8-B48F-1D18A9856A87 Silvia Caballero Mancebo author 2F1E1758-F248-11E8-B48F-1D18A9856A870000-0002-5223-3346 Gabriel Krens author 2B819732-F248-11E8-B48F-1D18A9856A870000-0003-4761-5996 Walter Kaufmann author 3F99E422-F248-11E8-B48F-1D18A9856A870000-0001-9735-5315 Karla Huljev author 44C6F6A6-F248-11E8-B48F-1D18A9856A87 Carl-Philipp J Heisenberg author 39427864-F248-11E8-B48F-1D18A9856A870000-0002-0912-4566 CaHe department EM-Fac department Bio department International IST Postdoc Fellowship Programme project Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation project Modulation of adhesion function in cell-cell contact formation by cortical tension project Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase, and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension stabilizing E-cadherin-actin complexes at the contact. 2020 eng bioRxiv10.1101/2020.11.20.391284 41 https://research-explorer.ista.ac.at/record/10766 https://research-explorer.ista.ac.at/record/9623 Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. 2020. doi:<a href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a> Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K, Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv, <a href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a>. Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv, 2020. <a href="https://doi.org/10.1101/2020.11.20.391284">https://doi.org/10.1101/2020.11.20.391284</a>. Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv, 2020, doi:<a href="https://doi.org/10.1101/2020.11.20.391284">10.1101/2020.11.20.391284</a>. Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W., Huljev, K., & Heisenberg, C.-P. J. (2020). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. <a href="https://doi.org/10.1101/2020.11.20.391284">https://doi.org/10.1101/2020.11.20.391284</a> J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K. Huljev, C.-P.J. Heisenberg, BioRxiv (2020). J. Slovakova et al., “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion,” bioRxiv. 2020. 97502021-07-29T11:29:50Z2026-07-11T22:31:13Z