---
_id: '9804'
abstract:
- lang: eng
text: Evolutionary studies are often limited by missing data that are critical to
understanding the history of selection. Selection experiments, which reproduce
rapid evolution under controlled conditions, are excellent tools to study how
genomes evolve under selection. Here we present a genomic dissection of the Longshanks
selection experiment, in which mice were selectively bred over 20 generations
for longer tibiae relative to body mass, resulting in 13% longer tibiae in two
replicates. We synthesized evolutionary theory, genome sequences and molecular
genetics to understand the selection response and found that it involved both
polygenic adaptation and discrete loci of major effect, with the strongest loci
tending to be selected in parallel between replicates. We show that selection
may favor de-repression of bone growth through inactivating two limb enhancers
of an inhibitor, Nkx3-2. Our integrative genomic analyses thus show that it is
possible to connect individual base-pair changes to the overall selection response.
article_processing_charge: No
author:
- first_name: João Pl
full_name: Castro, João Pl
last_name: Castro
- first_name: Michelle N.
full_name: Yancoskie, Michelle N.
last_name: Yancoskie
- first_name: Marta
full_name: Marchini, Marta
last_name: Marchini
- first_name: Stefanie
full_name: Belohlavy, Stefanie
id: 43FE426A-F248-11E8-B48F-1D18A9856A87
last_name: Belohlavy
orcid: 0000-0002-9849-498X
- first_name: Layla
full_name: Hiramatsu, Layla
last_name: Hiramatsu
- first_name: Marek
full_name: Kučka, Marek
last_name: Kučka
- first_name: William H.
full_name: Beluch, William H.
last_name: Beluch
- first_name: Ronald
full_name: Naumann, Ronald
last_name: Naumann
- first_name: Isabella
full_name: Skuplik, Isabella
last_name: Skuplik
- first_name: John
full_name: Cobb, John
last_name: Cobb
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
- first_name: Campbell
full_name: Rolian, Campbell
last_name: Rolian
- first_name: Yingguang Frank
full_name: Chan, Yingguang Frank
last_name: Chan
citation:
ama: 'Castro JP, Yancoskie MN, Marchini M, et al. Data from: An integrative genomic
analysis of the Longshanks selection experiment for longer limbs in mice. 2019.
doi:10.5061/dryad.0q2h6tk'
apa: 'Castro, J. P., Yancoskie, M. N., Marchini, M., Belohlavy, S., Hiramatsu, L.,
Kučka, M., … Chan, Y. F. (2019). Data from: An integrative genomic analysis of
the Longshanks selection experiment for longer limbs in mice. Dryad. https://doi.org/10.5061/dryad.0q2h6tk'
chicago: 'Castro, João Pl, Michelle N. Yancoskie, Marta Marchini, Stefanie Belohlavy,
Layla Hiramatsu, Marek Kučka, William H. Beluch, et al. “Data from: An Integrative
Genomic Analysis of the Longshanks Selection Experiment for Longer Limbs in Mice.”
Dryad, 2019. https://doi.org/10.5061/dryad.0q2h6tk.'
ieee: 'J. P. Castro et al., “Data from: An integrative genomic analysis of
the Longshanks selection experiment for longer limbs in mice.” Dryad, 2019.'
ista: 'Castro JP, Yancoskie MN, Marchini M, Belohlavy S, Hiramatsu L, Kučka M, Beluch
WH, Naumann R, Skuplik I, Cobb J, Barton NH, Rolian C, Chan YF. 2019. Data from:
An integrative genomic analysis of the Longshanks selection experiment for longer
limbs in mice, Dryad, 10.5061/dryad.0q2h6tk.'
mla: 'Castro, João Pl, et al. Data from: An Integrative Genomic Analysis of the
Longshanks Selection Experiment for Longer Limbs in Mice. Dryad, 2019, doi:10.5061/dryad.0q2h6tk.'
short: J.P. Castro, M.N. Yancoskie, M. Marchini, S. Belohlavy, L. Hiramatsu, M.
Kučka, W.H. Beluch, R. Naumann, I. Skuplik, J. Cobb, N.H. Barton, C. Rolian, Y.F.
Chan, (2019).
date_created: 2021-08-06T11:52:54Z
date_published: 2019-06-06T00:00:00Z
date_updated: 2023-08-29T06:41:51Z
day: '06'
department:
- _id: NiBa
doi: 10.5061/dryad.0q2h6tk
main_file_link:
- open_access: '1'
url: https://doi.org/10.5061/dryad.0q2h6tk
month: '06'
oa: 1
oa_version: Published Version
publisher: Dryad
related_material:
record:
- id: '6713'
relation: used_in_publication
status: public
status: public
title: 'Data from: An integrative genomic analysis of the Longshanks selection experiment
for longer limbs in mice'
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...