article published yes Andrea Vega author Isabel Fredes author José O’Brien author Zhouxin Shen author Krisztina Ötvös author 29B901B0-F248-11E8-B48F-1D18A9856A870000-0002-5503-4983 Rashed Abualia author 4827E134-F248-11E8-B48F-1D18A9856A870000-0002-9357-9415 Eva Benková author 38F4F166-F248-11E8-B48F-1D18A9856A870000-0002-8510-9739 Steven P. Briggs author Rodrigo A. Gutiérrez author EvBe department GradSch department Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate. https://research-explorer.ista.ac.at/download/9913/10090/2021_EmboR_Vega.pdf application/pdfno https://creativecommons.org/licenses/by-nc-nd/4.0/ Wiley2021 eng 1469-221X 1469-3178 34357701 00068175420000110.15252/embr.202051813 229 https://research-explorer.ista.ac.at/record/10303 Vega, A., Fredes, I., O’Brien, J., Shen, Z., Ötvös, K., Abualia, R., … Gutiérrez, R. A. (2021). Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. Wiley. <a href="https://doi.org/10.15252/embr.202051813">https://doi.org/10.15252/embr.202051813</a> Vega, Andrea, Isabel Fredes, José O’Brien, Zhouxin Shen, Krisztina Ötvös, Rashed Abualia, Eva Benková, Steven P. Briggs, and Rodrigo A. Gutiérrez. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>. Wiley, 2021. <a href="https://doi.org/10.15252/embr.202051813">https://doi.org/10.15252/embr.202051813</a>. Vega, Andrea, et al. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>, vol. 22, no. 9, e51813, Wiley, 2021, doi:<a href="https://doi.org/10.15252/embr.202051813">10.15252/embr.202051813</a>. A. Vega <i>et al.</i>, “Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture,” <i>EMBO Reports</i>, vol. 22, no. 9. Wiley, 2021. A. Vega, I. Fredes, J. O’Brien, Z. Shen, K. Ötvös, R. Abualia, E. Benková, S.P. Briggs, R.A. Gutiérrez, EMBO Reports 22 (2021). Vega A, Fredes I, O’Brien J, et al. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. 2021;22(9). doi:<a href="https://doi.org/10.15252/embr.202051813">10.15252/embr.202051813</a> Vega A, Fredes I, O’Brien J, Shen Z, Ötvös K, Abualia R, Benková E, Briggs SP, Gutiérrez RA. 2021. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. EMBO Reports. 22(9), e51813. 99132021-08-15T22:01:30Z2026-09-04T22:30:52Z