Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
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Abstract
Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021).
Publishing Year
Date Published
2021-12-17
Journal Title
STAR Protocols
Publisher
Elsevier
Acknowledgement
This research was supported by the European Research Council (grant 715571 to S.S.). We thank Rouven Schulz, Michael Schunn, Claudia Gold, Gabriel Krens, Sarah Gorkiewicz, Margaret Maes, Jürgen Siegert, Marco Benevento, and Sara Oakeley for comments on the manuscript and the IST Austria Bioimaging Facility for the technical support.
Acknowledged SSUs
Volume
2
Issue
4
Article Number
101012
eISSN
IST-REx-ID
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2021_STARProt_Venturino.pdf
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Open Access
Date Uploaded
2021-12-20
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