Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation
Anderson DJ, Hetzer M. 2008. Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation. Journal of Cell Biology. 182(5), 911–924.
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Journal Article
| Published
| English
Scopus indexed
Author
Anderson, Daniel J.;
Hetzer, MartinISTA
Corresponding author has ISTA affiliation
Abstract
During mitosis in metazoans, segregated chromosomes become enclosed by the nuclear envelope (NE), a double membrane that is continuous with the endoplasmic reticulum (ER). Recent in vitro data suggest that NE formation occurs by chromatin-mediated reorganization of the tubular ER; however, the basic principles of such a membrane-reshaping process remain uncharacterized. Here, we present a quantitative analysis of nuclear membrane assembly in mammalian cells using time-lapse microscopy. From the initial recruitment of ER tubules to chromatin, the formation of a membrane-enclosed, transport-competent nucleus occurs within ∼12 min. Overexpression of the ER tubule-forming proteins reticulon 3, reticulon 4, and DP1 inhibits NE formation and nuclear expansion, whereas their knockdown accelerates nuclear assembly. This suggests that the transition from membrane tubules to sheets is rate-limiting for nuclear assembly. Our results provide evidence that ER-shaping proteins are directly involved in the reconstruction of the nuclear compartment and that morphological restructuring of the ER is the principal mechanism of NE formation in vivo.
Keywords
Publishing Year
Date Published
2008-09-08
Journal Title
Journal of Cell Biology
Publisher
Rockefeller University Press
Volume
182
Issue
5
Page
911-924
ISSN
eISSN
IST-REx-ID
Cite this
Anderson DJ, Hetzer M. Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation. Journal of Cell Biology. 2008;182(5):911-924. doi:10.1083/jcb.200805140
Anderson, D. J., & Hetzer, M. (2008). Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.200805140
Anderson, Daniel J., and Martin Hetzer. “Reshaping of the Endoplasmic Reticulum Limits the Rate for Nuclear Envelope Formation.” Journal of Cell Biology. Rockefeller University Press, 2008. https://doi.org/10.1083/jcb.200805140.
D. J. Anderson and M. Hetzer, “Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation,” Journal of Cell Biology, vol. 182, no. 5. Rockefeller University Press, pp. 911–924, 2008.
Anderson DJ, Hetzer M. 2008. Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation. Journal of Cell Biology. 182(5), 911–924.
Anderson, Daniel J., and Martin Hetzer. “Reshaping of the Endoplasmic Reticulum Limits the Rate for Nuclear Envelope Formation.” Journal of Cell Biology, vol. 182, no. 5, Rockefeller University Press, 2008, pp. 911–24, doi:10.1083/jcb.200805140.
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PMID: 18779370
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