A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy
Michalska JM. 2023. A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy. Institute of Science and Technology Austria.
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Thesis
| PhD
| Published
| English
Author
Supervisor
Department
Series Title
ISTA Thesis
Abstract
The brain is an exceptionally sophisticated organ consisting of billions of cells and trillions of
connections that orchestrate our cognition and behavior. To decode its complex connectivity, it is
pivotal to disentangle its intricate architecture spanning from cm-sized circuits down to tens of
nm-small synapses.
To achieve this goal, I developed CATS – Comprehensive Analysis of nervous Tissue across
Scales, a versatile toolbox for obtaining a holistic view of nervous tissue context with (superresolution) fluorescence microscopy. CATS combines comprehensive labeling of the extracellular
space, that is compatible with chemical fixation, with information on molecular markers, superresolved data acquisition and machine-learning based data analysis for segmentation and synapse
identification.
I used CATS to analyze key features of nervous tissue connectivity, ranging from whole tissue
architecture, neuronal in- and output-fields, down to synapse morphology.
Focusing on the hippocampal circuitry, I quantified synaptic transmission properties of mossy
fiber boutons and analyzed the connectivity pattern of dentate gyrus granule cells with CA3
pyramidal neurons. This shows that CATS is a viable tool to study hallmarks of neuronal
connectivity with light microscopy.
Publishing Year
Date Published
2023-01-09
Acknowledged SSUs
Page
201
ISBN
ISSN
IST-REx-ID
Cite this
Michalska JM. A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy. 2023. doi:10.15479/at:ista:12470
Michalska, J. M. (2023). A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12470
Michalska, Julia M. “A Versatile Toolbox for the Comprehensive Analysis of Nervous Tissue Organization with Light Microscopy.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12470.
J. M. Michalska, “A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy,” Institute of Science and Technology Austria, 2023.
Michalska JM. 2023. A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy. Institute of Science and Technology Austria.
Michalska, Julia M. A Versatile Toolbox for the Comprehensive Analysis of Nervous Tissue Organization with Light Microscopy. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12470.
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