Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes

Praetorius FM, Dietz H. 2017. Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. Science. 355(6331), eaam5488.

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Journal Article | Published | English

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Author
Praetorius, Florian MISTA; Dietz, Hendrik
Abstract
We describe an approach to bottom-up fabrication that allows integration of the functional diversity of proteins into designed three-dimensional structural frameworks. A set of custom staple proteins based on transcription activator–like effector proteins folds a double-stranded DNA template into a user-defined shape. Each staple protein is designed to recognize and closely link two distinct double-helical DNA sequences at separate positions on the template. We present design rules for constructing megadalton-scale DNA-protein hybrid shapes; introduce various structural motifs, such as custom curvature, corners, and vertices; and describe principles for creating multilayer DNA-protein objects with enhanced rigidity. We demonstrate self-assembly of our hybrid nanostructures in one-pot mixtures that include the genetic information for the designed proteins, the template DNA, RNA polymerase, ribosomes, and cofactors for transcription and translation.
Publishing Year
Date Published
2017-03-24
Journal Title
Science
Volume
355
Issue
6331
Article Number
eaam5488
ISSN
eISSN
IST-REx-ID

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Praetorius FM, Dietz H. Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. Science. 2017;355(6331). doi:10.1126/science.aam5488
Praetorius, F. M., & Dietz, H. (2017). Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aam5488
Praetorius, Florian M, and Hendrik Dietz. “Self-Assembly of Genetically Encoded DNA-Protein Hybrid Nanoscale Shapes.” Science. American Association for the Advancement of Science, 2017. https://doi.org/10.1126/science.aam5488.
F. M. Praetorius and H. Dietz, “Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes,” Science, vol. 355, no. 6331. American Association for the Advancement of Science, 2017.
Praetorius FM, Dietz H. 2017. Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. Science. 355(6331), eaam5488.
Praetorius, Florian M., and Hendrik Dietz. “Self-Assembly of Genetically Encoded DNA-Protein Hybrid Nanoscale Shapes.” Science, vol. 355, no. 6331, eaam5488, American Association for the Advancement of Science, 2017, doi:10.1126/science.aam5488.

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