A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome

Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW. 2022. A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome. Nature Structural & Molecular Biology. 30, 91–98.

Download (ext.)

Journal Article | Published | English

Scopus indexed
Author
Yelland, James N.; Bravo, JackISTA ; Black, Joshua J.; Taylor, David W.; Johnson, Arlen W.
Abstract
RNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit, gates ribosome assembly. Massively parallel mutational scanning of the essential nuclear GTPase Nog2 identified important interactions with rRNA, particularly with the 2′-<jats:italic>O</jats:italic>-methylated A-site base Gm2922. We found that methylation of G2922 is needed for assembly and efficient nuclear export of the large subunit. Critically, we identified single amino acid changes in Nog2 that completely bypass dependence on G2922 methylation and used cryoelectron microscopy to directly visualize how methylation flips Gm2922 into the active site channel of Nog2. This work demonstrates that a single RNA modification is a critical checkpoint in ribosome biogenesis, suggesting that such modifications can play an important role in regulation and assembly of macromolecular machines.
Publishing Year
Date Published
2022-12-19
Journal Title
Nature Structural & Molecular Biology
Volume
30
Page
91-98
ISSN
eISSN
IST-REx-ID

Cite this

Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW. A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome. Nature Structural & Molecular Biology. 2022;30:91-98. doi:10.1038/s41594-022-00891-8
Yelland, J. N., Bravo, J. P. K., Black, J. J., Taylor, D. W., & Johnson, A. W. (2022). A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome. Nature Structural & Molecular Biology. Springer Nature. https://doi.org/10.1038/s41594-022-00891-8
Yelland, James N., Jack Peter Kelly Bravo, Joshua J. Black, David W. Taylor, and Arlen W. Johnson. “A Single 2′-O-Methylation of Ribosomal RNA Gates Assembly of a Functional Ribosome.” Nature Structural & Molecular Biology. Springer Nature, 2022. https://doi.org/10.1038/s41594-022-00891-8.
J. N. Yelland, J. P. K. Bravo, J. J. Black, D. W. Taylor, and A. W. Johnson, “A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome,” Nature Structural & Molecular Biology, vol. 30. Springer Nature, pp. 91–98, 2022.
Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW. 2022. A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome. Nature Structural & Molecular Biology. 30, 91–98.
Yelland, James N., et al. “A Single 2′-O-Methylation of Ribosomal RNA Gates Assembly of a Functional Ribosome.” Nature Structural & Molecular Biology, vol. 30, Springer Nature, 2022, pp. 91–98, doi:10.1038/s41594-022-00891-8.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]

Link(s) to Main File(s)
Access Level
OA Open Access

Export

Marked Publications

Open Data ISTA Research Explorer

Sources

PMID: 36536102
PubMed | Europe PMC

Search this title in

Google Scholar