Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf
Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology, vol. 2787, 305–313.
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Book Chapter
| Published
| English
Scopus indexed
Author
Jayasree, Aswathy;
Salava, Hymavathi;
Nodzynski, Tomasz;
Sravankumar, ThulaISTA 
Book Editor
Maghuly, Fatemeh
Department
Series Title
Methods in Molecular Biology
Abstract
Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.
Publishing Year
Date Published
2024-04-25
Book Title
Plant Functional Genomics
Publisher
Springer Nature
Acknowledgement
Special thanks to Dr. Marta Zwiewka for the support. Thanks to the Czech Science Foundation GA 20-20860Y for financial aid and support of A.S.S., respectively. Thanks go to Core Facility Cellular Imaging (CELLIM), and Plant Sciences Core Facility of CEITEC Masaryk University is acknowledged for the technical support.
Volume
2787
Page
305-313
ISBN
eISSN
IST-REx-ID
Cite this
Jayasree A, Salava H, Nodzynski T, Sravankumar T. Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Maghuly F, ed. Plant Functional Genomics. Vol 2787. MIMB. Springer Nature; 2024:305-313. doi:10.1007/978-1-0716-3778-4_21
Jayasree, A., Salava, H., Nodzynski, T., & Sravankumar, T. (2024). Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In F. Maghuly (Ed.), Plant Functional Genomics (Vol. 2787, pp. 305–313). Springer Nature. https://doi.org/10.1007/978-1-0716-3778-4_21
Jayasree, Aswathy, Hymavathi Salava, Tomasz Nodzynski, and Thula Sravankumar. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” In Plant Functional Genomics, edited by Fatemeh Maghuly, 2787:305–13. MIMB. Springer Nature, 2024. https://doi.org/10.1007/978-1-0716-3778-4_21.
A. Jayasree, H. Salava, T. Nodzynski, and T. Sravankumar, “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf,” in Plant Functional Genomics, vol. 2787, F. Maghuly, Ed. Springer Nature, 2024, pp. 305–313.
Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology, vol. 2787, 305–313.
Jayasree, Aswathy, et al. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” Plant Functional Genomics, edited by Fatemeh Maghuly, vol. 2787, Springer Nature, 2024, pp. 305–13, doi:10.1007/978-1-0716-3778-4_21.
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