PSME3 regulates migration and differentiation of myoblasts
Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 8(9), e202503208.
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Corresponding author has ISTA affiliation
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Abstract
The acquisition of cellular identity requires large-scale alterations in cellular state. The noncanonical proteasome activator PSME3 is known to regulate diverse cellular processes, but its importance for differentiation remains unclear. Here, we demonstrate that PSME3 binds dynamically to highly active promoters over the course of differentiation. However, loss of PSME3 does not globally affect mRNA transcription. We find instead that PSME3 influences the levels of several adhesion-related proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility and myoblast differentiation in a proteasome-independent manner. Our findings reveal several new facets of PSME3 functionality and highlight its importance for the differentiation of myogenic cells.
Publishing Year
Date Published
2025-06-19
Journal Title
Life Science Alliance
Publisher
Embo Press
Acknowledgement
All proteomics analysis was done by the ISTA LSF Mass Spectrometry Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup); Bella Bruszel optimized the acquisition methods, acquired the data, and performed all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed the search results using a development version of their data analysis package, proteoCraft (publication pending). The authors would like to thank Saki for their clarity of thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory for invaluable discussions relating to the project. This research was further supported by the Lab Support Facility and the Imaging and Optics Facility of ISTA.
Acknowledged SSUs
Volume
8
Issue
9
Article Number
e202503208
eISSN
IST-REx-ID
Cite this
Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 2025;8(9). doi:10.26508/lsa.202503208
Kuhn, K. D., Cho, U. H., & Hetzer, M. (2025). PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. Embo Press. https://doi.org/10.26508/lsa.202503208
Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration and Differentiation of Myoblasts.” Life Science Alliance. Embo Press, 2025. https://doi.org/10.26508/lsa.202503208.
K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation of myoblasts,” Life Science Alliance, vol. 8, no. 9. Embo Press, 2025.
Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 8(9), e202503208.
Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of Myoblasts.” Life Science Alliance, vol. 8, no. 9, e202503208, Embo Press, 2025, doi:10.26508/lsa.202503208.
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