Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide
Hashimoto H, Ishihara T, Shigemoto R, Mori K, Nagata S. 1993. Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide. Neuron. 11(2), 333–342.
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Journal Article
| Published
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Author
Hashimoto, Hitoshi;
Ishihara, Takeshi;
Shigemoto, RyuichiISTA 
;
Mori, Kensaku;
Nagata, Shigekazu
;
Mori, Kensaku;
Nagata, ShigekazuAbstract
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a polypeptide hormone related to vasoactive intestinal polypeptide (VIP). Rat PACAP receptor cDNA was isolated from a brain cDNA library by cross-hybridization with rat VIP receptor cDNA. The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000 times higher affinity than VIP, and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The rat PACAP receptor consists of 495 amino acids, contains seven transmembrane segments, and has a significant similarity with other Gs-coupled receptors, such as VIP, glucagon, and secretin receptors. PACAP receptor mRNA was abundantly expressed in the brain, but not in the peripheral tissues except for the adrenal gland. In situ hybridization revealed a high level of expression of PACAP receptor mRNA in the hippocampal dentate gyrus, olfactory bulb, and cerebellar cortex.
Publishing Year
Date Published
1993-08-01
Journal Title
Neuron
Publisher
Elsevier
Acknowledgement
We are grateful to Drs. Y. Sugimoto, A. Ichikawa, J. Ogasawara, R. Fukunaga, H. Aino, and A. Baba for discussion and advice. We also thank Ms. K. Mimura for secretarial assistance. This work was supported in part by the Ministry of Education, Science and Culture of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.
Volume
11
Issue
2
Page
333 - 342
ISSN
IST-REx-ID
Cite this
Hashimoto H, Ishihara T, Shigemoto R, Mori K, Nagata S. Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide. Neuron. 1993;11(2):333-342. doi:10.1016/0896-6273(93)90188-W
Hashimoto, H., Ishihara, T., Shigemoto, R., Mori, K., & Nagata, S. (1993). Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide. Neuron. Elsevier. https://doi.org/10.1016/0896-6273(93)90188-W
Hashimoto, Hitoshi, Takeshi Ishihara, Ryuichi Shigemoto, Kensaku Mori, and Shigekazu Nagata. “ Molecular Cloning and Tissue Distribution of a Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide.” Neuron. Elsevier, 1993. https://doi.org/10.1016/0896-6273(93)90188-W.
H. Hashimoto, T. Ishihara, R. Shigemoto, K. Mori, and S. Nagata, “ Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide,” Neuron, vol. 11, no. 2. Elsevier, pp. 333–342, 1993.
Hashimoto H, Ishihara T, Shigemoto R, Mori K, Nagata S. 1993. Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide. Neuron. 11(2), 333–342.
Hashimoto, Hitoshi, et al. “ Molecular Cloning and Tissue Distribution of a Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide.” Neuron, vol. 11, no. 2, Elsevier, 1993, pp. 333–42, doi:10.1016/0896-6273(93)90188-W.
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