In situ hybridization for mRNA detection in Arabidopsis tissue sections
Brewer P, Heisler M, Hejátko J, Friml J, Benková E. 2006. In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols. 1(3), 1462–1467.
Download
No fulltext has been uploaded. References only!
Journal Article
| Published
Author
Abstract
Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.
Publishing Year
Date Published
2006-08-01
Journal Title
Nature Protocols
Publisher
Nature Publishing Group
Volume
1
Issue
3
Page
1462 - 1467
IST-REx-ID
Cite this
Brewer P, Heisler M, Hejátko J, Friml J, Benková E. In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols. 2006;1(3):1462-1467. doi:10.1038/nprot.2006.226
Brewer, P., Heisler, M., Hejátko, J., Friml, J., & Benková, E. (2006). In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.226
Brewer, Philip, Marcus Heisler, Jan Hejátko, Jiří Friml, and Eva Benková. “In Situ Hybridization for MRNA Detection in Arabidopsis Tissue Sections.” Nature Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.226.
P. Brewer, M. Heisler, J. Hejátko, J. Friml, and E. Benková, “In situ hybridization for mRNA detection in Arabidopsis tissue sections,” Nature Protocols, vol. 1, no. 3. Nature Publishing Group, pp. 1462–1467, 2006.
Brewer P, Heisler M, Hejátko J, Friml J, Benková E. 2006. In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols. 1(3), 1462–1467.
Brewer, Philip, et al. “In Situ Hybridization for MRNA Detection in Arabidopsis Tissue Sections.” Nature Protocols, vol. 1, no. 3, Nature Publishing Group, 2006, pp. 1462–67, doi:10.1038/nprot.2006.226.
Google Scholar