Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi
Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 1(4), 223–225.
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Journal Article
| Published
| English
Author
Conn, Jan;
Bollback, Jonathan PISTA 
;
Onyabe, David;
Robinson, Tessa;
Wilkerson, Richard;
Povoa, Marinete
;
Onyabe, David;
Robinson, Tessa;
Wilkerson, Richard;
Povoa, MarineteAbstract
High molecular weight DNA was extracted from the primary Neotropical malaria vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert genomic library, and then a phagemid library. Enriched sublibraries were constructed from the phagemid library using a microsatellite oligo primed second strand synthesis protocol. The resulting 242 760 individual clones were screened. The mean clone size of the positive clones was 302 bp. Flanking primers were designed for each suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized. The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian and central Brazil.
Publishing Year
Date Published
2001-12-01
Journal Title
Molecular Ecology Notes
Acknowledgement
For support in Brazil we thank D. Galiza, R.N.L. Lacerda,E.P. Santa Rosa, M.N.O. Segura, and R.T.L. de Souza. We also thankM.J. Braun for allowing work on the library construction at theLaboratory of Molecular Systematics, Washington. Supported byNIH AI 40116 to JEC and Instituto Evandro Chagas, Belém, Brazil.
Volume
1
Issue
4
Page
223 - 225
ISSN
IST-REx-ID
Cite this
Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 2001;1(4):223-225. doi: 10.1046/j.1471-8278.2001.00078.x
Conn, J., Bollback, J. P., Onyabe, D., Robinson, T., Wilkerson, R., & Povoa, M. (2001). Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. Wiley-Blackwell. https://doi.org/ 10.1046/j.1471-8278.2001.00078.x
Conn, Jan, Jonathan P Bollback, David Onyabe, Tessa Robinson, Richard Wilkerson, and Marinete Povoa. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes. Wiley-Blackwell, 2001. https://doi.org/ 10.1046/j.1471-8278.2001.00078.x.
J. Conn, J. P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, and M. Povoa, “Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi,” Molecular Ecology Notes, vol. 1, no. 4. Wiley-Blackwell, pp. 223–225, 2001.
Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 1(4), 223–225.
Conn, Jan, et al. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes, vol. 1, no. 4, Wiley-Blackwell, 2001, pp. 223–25, doi: 10.1046/j.1471-8278.2001.00078.x.
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