Imaging zebrafish embryos by two-photon excitation time-lapse microscopy
Carvalho L, Heisenberg C-PJ. 2010. Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. 546(Part 5), 273–287.
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Journal Article
| Published
| English
Author
Carvalho, Lara;
Heisenberg, Carl-Philipp ISTA 
Abstract
The zebrafish is a favorite model organism to study tissue morphogenesis during development at a subcellular level. This largely results from the fact that zebrafish embryos are transparent and thus accessible to various imaging techniques, such as confocal and two-photon excitation (2PE) microscopy. In particular, 2PE microscopy has been shown to be useful for imaging deep cell layers within the embryo and following tissue morphogenesis over long periods. This chapter describes how to use 2PE microscopy to study morphogenetic movements during early zebrafish embryonic development, providing a general blueprint for its use in zebrafish.
Publishing Year
Date Published
2010-01-01
Journal Title
Methods in Molecular Biology
Publisher
Springer
Volume
546
Issue
Part 5
Page
273 - 287
IST-REx-ID
Cite this
Carvalho L, Heisenberg C-PJ. Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. 2010;546(Part 5):273-287. doi:10.1007/978-1-60327-977-2_17
Carvalho, L., & Heisenberg, C.-P. J. (2010). Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. Springer. https://doi.org/10.1007/978-1-60327-977-2_17
Carvalho, Lara, and Carl-Philipp J Heisenberg. “Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy.” Methods in Molecular Biology. Springer, 2010. https://doi.org/10.1007/978-1-60327-977-2_17.
L. Carvalho and C.-P. J. Heisenberg, “Imaging zebrafish embryos by two-photon excitation time-lapse microscopy,” Methods in Molecular Biology, vol. 546, no. Part 5. Springer, pp. 273–287, 2010.
Carvalho L, Heisenberg C-PJ. 2010. Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. 546(Part 5), 273–287.
Carvalho, Lara, and Carl-Philipp J. Heisenberg. “Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy.” Methods in Molecular Biology, vol. 546, no. Part 5, Springer, 2010, pp. 273–87, doi:10.1007/978-1-60327-977-2_17.
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