Lifeact: a versatile marker to visualize F-actin
Riedl J, Crevenna A, Kessenbrock K, Yu J, Neukirchen D, Bista M, Bradke F, Jenne D, Holak T, Werb Z, Sixt MK, Wedlich Soldner R. 2008. Lifeact: a versatile marker to visualize F-actin. Nature Methods. 5(7), 605–607.
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Journal Article
| Published
Author
Riedl, Julia;
Crevenna, Alvaro H;
Kessenbrock, Kai;
Yu, Jerry Haochen;
Neukirchen, Dorothee;
Bista, Michal;
Bradke, Frank;
Jenne, Dieter;
Holak, Tad A;
Werb, Zena;
Sixt, Michael KISTA 
;
Wedlich-Soldner, Roland
All
;
Wedlich-Soldner, Roland
All
Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Publishing Year
Date Published
2008-06-08
Journal Title
Nature Methods
Volume
5
Issue
7
Page
605 - 607
IST-REx-ID
Cite this
Riedl J, Crevenna A, Kessenbrock K, et al. Lifeact: a versatile marker to visualize F-actin. Nature Methods. 2008;5(7):605-607. doi:10.1038/nmeth.1220
Riedl, J., Crevenna, A., Kessenbrock, K., Yu, J., Neukirchen, D., Bista, M., … Wedlich Soldner, R. (2008). Lifeact: a versatile marker to visualize F-actin. Nature Methods. Nature Publishing Group. https://doi.org/10.1038/nmeth.1220
Riedl, Julia, Alvaro Crevenna, Kai Kessenbrock, Jerry Yu, Dorothee Neukirchen, Michal Bista, Frank Bradke, et al. “Lifeact: A Versatile Marker to Visualize F-Actin.” Nature Methods. Nature Publishing Group, 2008. https://doi.org/10.1038/nmeth.1220.
J. Riedl et al., “Lifeact: a versatile marker to visualize F-actin,” Nature Methods, vol. 5, no. 7. Nature Publishing Group, pp. 605–607, 2008.
Riedl J, Crevenna A, Kessenbrock K, Yu J, Neukirchen D, Bista M, Bradke F, Jenne D, Holak T, Werb Z, Sixt MK, Wedlich Soldner R. 2008. Lifeact: a versatile marker to visualize F-actin. Nature Methods. 5(7), 605–607.
Riedl, Julia, et al. “Lifeact: A Versatile Marker to Visualize F-Actin.” Nature Methods, vol. 5, no. 7, Nature Publishing Group, 2008, pp. 605–07, doi:10.1038/nmeth.1220.
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