Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast
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Author
Lukačišin, MartinISTA
;
Landon, Matthieu;
Jajoo, Rishi

Department
Abstract
RNA Polymerase II pauses and backtracks during transcription, with many consequences for gene expression and cellular physiology. Here, we show that the energy required to melt double-stranded nucleic acids in the transcription bubble predicts pausing in Saccharomyces cerevisiae far more accurately than nucleosome roadblocks do. In addition, the same energy difference also determines when the RNA polymerase backtracks instead of continuing to move forward. This data-driven model corroborates—in a genome wide and quantitative manner—previous evidence that sequence-dependent thermodynamic features of nucleic acids influence both transcriptional pausing and backtracking.
Publishing Year
Date Published
2017-03-16
Journal Title
PLoS One
Publisher
Public Library of Science
Volume
12
Issue
3
Article Number
e0174066
ISSN
IST-REx-ID
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2018-12-12
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