Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication
Bravo JPK, Dangerfield TL, Taylor DW, Johnson KA. 2021. Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication. Molecular Cell. 81(7), 1548–1552.e4.
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Author
Bravo, JackISTA 
;
Dangerfield, Tyler L.;
Taylor, David W.;
Johnson, Kenneth A.
;
Dangerfield, Tyler L.;
Taylor, David W.;
Johnson, Kenneth A.Abstract
Remdesivir is a nucleoside analog approved by the US FDA for treatment of COVID-19. Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA-dependent RNA polymerase complex, revealing full incorporation of 3 copies of remdesivir monophosphate (RMP) and a partially incorporated fourth RMP in the active site. The structure reveals that RMP blocks RNA translocation after incorporation of 3 bases following RMP, resulting in delayed chain termination, which can guide the rational design of improved antiviral drugs.
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Publishing Year
Date Published
2021-04-01
Journal Title
Molecular Cell
Publisher
Elsevier
Volume
81
Issue
7
Page
1548-1552.e4
ISSN
IST-REx-ID
Cite this
Bravo JPK, Dangerfield TL, Taylor DW, Johnson KA. Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication. Molecular Cell. 2021;81(7):1548-1552.e4. doi:10.1016/j.molcel.2021.01.035
Bravo, J. P. K., Dangerfield, T. L., Taylor, D. W., & Johnson, K. A. (2021). Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication. Molecular Cell. Elsevier. https://doi.org/10.1016/j.molcel.2021.01.035
Bravo, Jack Peter Kelly, Tyler L. Dangerfield, David W. Taylor, and Kenneth A. Johnson. “Remdesivir Is a Delayed Translocation Inhibitor of SARS-CoV-2 Replication.” Molecular Cell. Elsevier, 2021. https://doi.org/10.1016/j.molcel.2021.01.035.
J. P. K. Bravo, T. L. Dangerfield, D. W. Taylor, and K. A. Johnson, “Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication,” Molecular Cell, vol. 81, no. 7. Elsevier, p. 1548–1552.e4, 2021.
Bravo JPK, Dangerfield TL, Taylor DW, Johnson KA. 2021. Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication. Molecular Cell. 81(7), 1548–1552.e4.
Bravo, Jack Peter Kelly, et al. “Remdesivir Is a Delayed Translocation Inhibitor of SARS-CoV-2 Replication.” Molecular Cell, vol. 81, no. 7, Elsevier, 2021, p. 1548–1552.e4, doi:10.1016/j.molcel.2021.01.035.
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