Structure of a type IV CRISPR-Cas ribonucleoprotein complex
Zhou Y, Bravo JPK, Taylor HN, Steens JA, Jackson RN, Staals RHJ, Taylor DW. 2021. Structure of a type IV CRISPR-Cas ribonucleoprotein complex. iScience. 24(3), 102201.
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Author
Zhou, Yi;
Bravo, JackISTA 
;
Taylor, Hannah N.;
Steens, Jurre A.;
Jackson, Ryan N.;
Staals, Raymond H.J.;
Taylor, David W.
;
Taylor, Hannah N.;
Steens, Jurre A.;
Jackson, Ryan N.;
Staals, Raymond H.J.;
Taylor, David W.Abstract
We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles the type III-A CRISPR Csm effector complex, consisting of a Cas7-like (Csf2) filament intertwined with a small subunit (Cas11) filament, but the complex lacks subunits for RNA processing and target DNA cleavage. Surprisingly, instead of assembling around a CRISPR-derived RNA (crRNA), the complex assembles upon heterogeneous RNA of a regular length arranged in a pseudo-A-form configuration. These findings provide a high-resolution glimpse into the assembly and function of enigmatic type IV CRISPR systems, expanding our understanding of class I CRISPR-Cas system architecture, and suggesting a function for type IV-B RNPs that may be distinct from other class 1 CRISPR-associated systems.
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Publishing Year
Date Published
2021-03-19
Journal Title
iScience
Publisher
Elsevier
Volume
24
Issue
3
Article Number
102201
ISSN
IST-REx-ID
Cite this
Zhou Y, Bravo JPK, Taylor HN, et al. Structure of a type IV CRISPR-Cas ribonucleoprotein complex. iScience. 2021;24(3). doi:10.1016/j.isci.2021.102201
Zhou, Y., Bravo, J. P. K., Taylor, H. N., Steens, J. A., Jackson, R. N., Staals, R. H. J., & Taylor, D. W. (2021). Structure of a type IV CRISPR-Cas ribonucleoprotein complex. IScience. Elsevier. https://doi.org/10.1016/j.isci.2021.102201
Zhou, Yi, Jack Peter Kelly Bravo, Hannah N. Taylor, Jurre A. Steens, Ryan N. Jackson, Raymond H.J. Staals, and David W. Taylor. “Structure of a Type IV CRISPR-Cas Ribonucleoprotein Complex.” IScience. Elsevier, 2021. https://doi.org/10.1016/j.isci.2021.102201.
Y. Zhou et al., “Structure of a type IV CRISPR-Cas ribonucleoprotein complex,” iScience, vol. 24, no. 3. Elsevier, 2021.
Zhou Y, Bravo JPK, Taylor HN, Steens JA, Jackson RN, Staals RHJ, Taylor DW. 2021. Structure of a type IV CRISPR-Cas ribonucleoprotein complex. iScience. 24(3), 102201.
Zhou, Yi, et al. “Structure of a Type IV CRISPR-Cas Ribonucleoprotein Complex.” IScience, vol. 24, no. 3, 102201, Elsevier, 2021, doi:10.1016/j.isci.2021.102201.
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