Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import

Khalfaoui-Hassani, Bahia; Trasnea, Petru Iulian; Steimle, Stefan; Koch, Hans-Georg; Daldal, Fevzi

Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import

Khalfaoui-Hassani B, Trasnea PI, Steimle S, Koch H-G, Daldal F. 2021. Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import. mBio. 12(4), e01567.

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2021_mBio_KhalfaouiHassani.pdf 3.38 MB

DOI

10.1128/mbio.01567-21

Journal Article | Published | English

Author

Khalfaoui-Hassani, Bahia; Trasnea, Petru Iulian^ISTA; Steimle, Stefan; Koch, Hans-Georg; Daldal, Fevzi

Department

Sazanov Group

Abstract

Copper (Cu) is a redox-active micronutrient that is both essential and toxic. Its cellular homeostasis is critical for supporting cuproprotein maturation while avoiding excessive oxidative stress. The Cu importer CcoA is the prototype of the widespread CalT subfamily of the MFS-type transporters. Hence, understanding its molecular mechanism of function is significant. Here, we show that CcoA undergoes a thiol:disulfide oxidoreduction cycle, which is important for its Cu import activity.

Keywords

Virology; Microbiology

Publishing Year

2021

Date Published

2021-08-31

Journal Title

mBio

Volume

Issue

Article Number

e01567

ISSN

2150-7511

IST-REx-ID

15274

Cite this

Khalfaoui-Hassani B, Trasnea PI, Steimle S, Koch H-G, Daldal F. Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import. mBio. 2021;12(4). doi:10.1128/mbio.01567-21

Khalfaoui-Hassani, B., Trasnea, P. I., Steimle, S., Koch, H.-G., & Daldal, F. (2021). Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import. MBio. American Society for Microbiology. https://doi.org/10.1128/mbio.01567-21

Khalfaoui-Hassani, Bahia, Petru Iulian Trasnea, Stefan Steimle, Hans-Georg Koch, and Fevzi Daldal. “Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import.” MBio. American Society for Microbiology, 2021. https://doi.org/10.1128/mbio.01567-21.

B. Khalfaoui-Hassani, P. I. Trasnea, S. Steimle, H.-G. Koch, and F. Daldal, “Cysteine mutants of the major facilitator superfamily-type transporter CcoA provide insight into copper import,” mBio, vol. 12, no. 4. American Society for Microbiology, 2021.

Khalfaoui-Hassani, Bahia, et al. “Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import.” MBio, vol. 12, no. 4, e01567, American Society for Microbiology, 2021, doi:10.1128/mbio.01567-21.

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