DNA Cloning
Watson J, Arroyo-Urea S, García-Nafría J. 2024.DNA Cloning. In: Handbook of Molecular Biotechnology. , 66–72.
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Book Chapter
| Published
| English
Scopus indexed
Author
Watson, JakeISTA
;
Arroyo-Urea, Sandra;
García-Nafría, Javier
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Book Editor
Liu, Dongyou
Department
Abstract
DNA cloning is a core technique in biomedical and biotechnological research and is used to assemble and modify DNA fragments at will. While DNA cloning has traditionally relied on restriction enzymes, recent homology-based methods offer improved protocols together with seamless and directional assembly of desired products, overcoming the main disadvantages of restriction enzyme DNA cloning. This chapter provides a historical perspective on DNA cloning, presents a detailed discussion on state-of-the-art in vitro and in vivo homology-based methodologies, covering the basics of how to perform all major plasmid modifications (sub-cloning, site-directed mutagenesis, insertions, and deletions), and gives examples of how to apply these techniques for complex DNA cloning projects.
Publishing Year
Date Published
2024-09-05
Book Title
Handbook of Molecular Biotechnology
Page
66-72
IST-REx-ID
Cite this
Watson J, Arroyo-Urea S, García-Nafría J. DNA Cloning. In: Liu D, ed. Handbook of Molecular Biotechnology. 1st ed. Boca Raton: CRC Press; 2024:66-72. doi:10.1201/9781003055211-8
Watson, J., Arroyo-Urea, S., & García-Nafría, J. (2024). DNA Cloning. In D. Liu (Ed.), Handbook of Molecular Biotechnology (1st ed., pp. 66–72). Boca Raton: CRC Press. https://doi.org/10.1201/9781003055211-8
Watson, Jake, Sandra Arroyo-Urea, and Javier García-Nafría. “DNA Cloning.” In Handbook of Molecular Biotechnology, edited by Dongyou Liu, 1st ed., 66–72. Boca Raton: CRC Press, 2024. https://doi.org/10.1201/9781003055211-8.
J. Watson, S. Arroyo-Urea, and J. García-Nafría, “DNA Cloning,” in Handbook of Molecular Biotechnology, 1st ed., D. Liu, Ed. Boca Raton: CRC Press, 2024, pp. 66–72.
Watson J, Arroyo-Urea S, García-Nafría J. 2024.DNA Cloning. In: Handbook of Molecular Biotechnology. , 66–72.
Watson, Jake, et al. “DNA Cloning.” Handbook of Molecular Biotechnology, edited by Dongyou Liu, 1st ed., CRC Press, 2024, pp. 66–72, doi:10.1201/9781003055211-8.