Protein expression enhancement in efflux-deleted mutant bacteria
Le T, Guet CC, Cluzel P. 2006. Protein expression enhancement in efflux-deleted mutant bacteria. Protein Expression and Purification. 48(1), 28–31.
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Journal Article
| Published
Author
Le,Thuc T.;
Guet, Calin CISTA 
;
Cluzel,Philippe
;
Cluzel,PhilippeAbstract
We applied a single-cell assay to characterize how transcription dynamics affects protein expression levels of a tetracycline-inducible gene expression system. Transcriptional activity of the tetracycline promoter in response to a steady level of inducer is steady in ΔacrAB efflux mutant but pulsating in wildtype Escherichia coli cells. We found that the expression level of the green fluorescent protein is several folds higher in ΔacrAB efflux mutant than in wildtype cells.
Publishing Year
Date Published
2006-07-01
Journal Title
Protein Expression and Purification
Publisher
Elsevier
Volume
48
Issue
1
Page
28 - 31
IST-REx-ID
Cite this
Le T, Guet CC, Cluzel P. Protein expression enhancement in efflux-deleted mutant bacteria. Protein Expression and Purification. 2006;48(1):28-31.
Le, T., Guet, C. C., & Cluzel, P. (2006). Protein expression enhancement in efflux-deleted mutant bacteria. Protein Expression and Purification. Elsevier.
Le, Thuc, Calin C Guet, and Philippe Cluzel. “Protein Expression Enhancement in Efflux-Deleted Mutant Bacteria.” Protein Expression and Purification. Elsevier, 2006.
T. Le, C. C. Guet, and P. Cluzel, “Protein expression enhancement in efflux-deleted mutant bacteria,” Protein Expression and Purification, vol. 48, no. 1. Elsevier, pp. 28–31, 2006.
Le T, Guet CC, Cluzel P. 2006. Protein expression enhancement in efflux-deleted mutant bacteria. Protein Expression and Purification. 48(1), 28–31.
Le, Thuc, et al. “Protein Expression Enhancement in Efflux-Deleted Mutant Bacteria.” Protein Expression and Purification, vol. 48, no. 1, Elsevier, 2006, pp. 28–31.
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